Viticulture is one of the agricultural sectors with major economic importance in Mediterranean climate zones. Salinity is considered a substantial issue for agricultural sectors in arid and semi-arid regions, where it has the potential to impair production of grape (Vitis vinifera), which is categorize as a moderately sensitive species to salinity, and its impact is expected to increase with climate change. As exploiting genetic diversity is one of the most promising strategies to cope with the negative impacts of climate change on viticulture and adapt under the new conditions to maintain grape production and quality, the study aimed to explore salinity tolerance mechanisms in diverse Egyptian grape genotypes based on morpho-physiological, biochemical, anatomical and gene expression analysis. Nine local grape genotypes; Baltim Eswid, Edkawy, Matrouh Eswid, Bez El-Naka, Bez El-Anza, Romy Ahmer, Gharibi, Fayoumi and Romy Abiad, were evaluated for tolerance under saline conditions. Salinity stress was induced in three levels of 2.28, 3.75 and 5.20 ms (using NaCl at 1000, 2000 and 3000 ppm, respectively) comparing to 695 µs irrigation water as control. Results indicated that, the growth of all investigated local grape genotypes was adversely affected by salt treatments in a cultivar-dependent manner. Proposed salt-tolerance mechanisms including; controlling the growth rate, reducing damage resulting from oxidative stress associated with salinity, keep balanced hydric status, structural alterations allowing protection and regulation of ions uptake. It was observed that, Edkawy local grape cultivar is a promising salt-tolerant genotype. On the other hand, Romy Abiad and Gharibi genotypes were classified as the most salt-sensitive comparable with other tested local cultivars. Bez El-Anza genotype which maintained 100% survivability under severe salinity stress condition was characterized by a remarkable decline in vegetative growth accompanied with keeping more leaves with a marked reduction in leaf area and most measurements of certain anatomical features, slight Na uptake, but undisputed oxidative stress indicators and down-regulated expression folds of AREB2 transcription factor; a sugar accumulation regulatory related gene. Therefore, local genotypes of Egyptian table grapes can be considered a storehouse of germplasm that should be conserved and not threatened with extinction or complete loss because they are adapted to severe environmental conditions and harsh cultural managements.
| Published in | American Journal of BioScience (Volume 11, Issue 6) |
| DOI | 10.11648/j.ajbio.20231106.16 |
| Page(s) | 171-186 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2023. Published by Science Publishing Group |
Grape, Salinity, Genotypes, Oxidative Stress, Biochemical, Anatomical, Gene Expression
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APA Style
Mahmoud, R., Dahab, A., Mahmoud, G., El-Wahab, M. A., Ismail, A., et al. (2023). Exploring Salinity Tolerance Mechanisms in Diverse Egyptian Grape Genotypes Based on Morpho-Physiological, Biochemical, Anatomical and Gene Expression Analysis. American Journal of BioScience, 11(6), 171-186. https://doi.org/10.11648/j.ajbio.20231106.16
ACS Style
Mahmoud, R.; Dahab, A.; Mahmoud, G.; El-Wahab, M. A.; Ismail, A., et al. Exploring Salinity Tolerance Mechanisms in Diverse Egyptian Grape Genotypes Based on Morpho-Physiological, Biochemical, Anatomical and Gene Expression Analysis. Am. J. BioScience 2023, 11(6), 171-186. doi: 10.11648/j.ajbio.20231106.16
AMA Style
Mahmoud R, Dahab A, Mahmoud G, El-Wahab MA, Ismail A, et al. Exploring Salinity Tolerance Mechanisms in Diverse Egyptian Grape Genotypes Based on Morpho-Physiological, Biochemical, Anatomical and Gene Expression Analysis. Am J BioScience. 2023;11(6):171-186. doi: 10.11648/j.ajbio.20231106.16
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Download@article{10.11648/j.ajbio.20231106.16,
author = {Rania Mahmoud and Abeer Dahab and Gehan Mahmoud and Mohamed Abd El-Wahab and Ahmed Ismail and Ali Farsson},
title = {Exploring Salinity Tolerance Mechanisms in Diverse Egyptian Grape Genotypes Based on Morpho-Physiological, Biochemical, Anatomical and Gene Expression Analysis},
journal = {American Journal of BioScience},
volume = {11},
number = {6},
pages = {171-186},
doi = {10.11648/j.ajbio.20231106.16},
url = {https://doi.org/10.11648/j.ajbio.20231106.16},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20231106.16},
abstract = {Viticulture is one of the agricultural sectors with major economic importance in Mediterranean climate zones. Salinity is considered a substantial issue for agricultural sectors in arid and semi-arid regions, where it has the potential to impair production of grape (Vitis vinifera), which is categorize as a moderately sensitive species to salinity, and its impact is expected to increase with climate change. As exploiting genetic diversity is one of the most promising strategies to cope with the negative impacts of climate change on viticulture and adapt under the new conditions to maintain grape production and quality, the study aimed to explore salinity tolerance mechanisms in diverse Egyptian grape genotypes based on morpho-physiological, biochemical, anatomical and gene expression analysis. Nine local grape genotypes; Baltim Eswid, Edkawy, Matrouh Eswid, Bez El-Naka, Bez El-Anza, Romy Ahmer, Gharibi, Fayoumi and Romy Abiad, were evaluated for tolerance under saline conditions. Salinity stress was induced in three levels of 2.28, 3.75 and 5.20 ms (using NaCl at 1000, 2000 and 3000 ppm, respectively) comparing to 695 µs irrigation water as control. Results indicated that, the growth of all investigated local grape genotypes was adversely affected by salt treatments in a cultivar-dependent manner. Proposed salt-tolerance mechanisms including; controlling the growth rate, reducing damage resulting from oxidative stress associated with salinity, keep balanced hydric status, structural alterations allowing protection and regulation of ions uptake. It was observed that, Edkawy local grape cultivar is a promising salt-tolerant genotype. On the other hand, Romy Abiad and Gharibi genotypes were classified as the most salt-sensitive comparable with other tested local cultivars. Bez El-Anza genotype which maintained 100% survivability under severe salinity stress condition was characterized by a remarkable decline in vegetative growth accompanied with keeping more leaves with a marked reduction in leaf area and most measurements of certain anatomical features, slight Na uptake, but undisputed oxidative stress indicators and down-regulated expression folds of AREB2 transcription factor; a sugar accumulation regulatory related gene. Therefore, local genotypes of Egyptian table grapes can be considered a storehouse of germplasm that should be conserved and not threatened with extinction or complete loss because they are adapted to severe environmental conditions and harsh cultural managements.
},
year = {2023}
}
TY - JOUR T1 - Exploring Salinity Tolerance Mechanisms in Diverse Egyptian Grape Genotypes Based on Morpho-Physiological, Biochemical, Anatomical and Gene Expression Analysis AU - Rania Mahmoud AU - Abeer Dahab AU - Gehan Mahmoud AU - Mohamed Abd El-Wahab AU - Ahmed Ismail AU - Ali Farsson Y1 - 2023/12/22 PY - 2023 N1 - https://doi.org/10.11648/j.ajbio.20231106.16 DO - 10.11648/j.ajbio.20231106.16 T2 - American Journal of BioScience JF - American Journal of BioScience JO - American Journal of BioScience SP - 171 EP - 186 PB - Science Publishing Group SN - 2330-0167 UR - https://doi.org/10.11648/j.ajbio.20231106.16 AB - Viticulture is one of the agricultural sectors with major economic importance in Mediterranean climate zones. Salinity is considered a substantial issue for agricultural sectors in arid and semi-arid regions, where it has the potential to impair production of grape (Vitis vinifera), which is categorize as a moderately sensitive species to salinity, and its impact is expected to increase with climate change. As exploiting genetic diversity is one of the most promising strategies to cope with the negative impacts of climate change on viticulture and adapt under the new conditions to maintain grape production and quality, the study aimed to explore salinity tolerance mechanisms in diverse Egyptian grape genotypes based on morpho-physiological, biochemical, anatomical and gene expression analysis. Nine local grape genotypes; Baltim Eswid, Edkawy, Matrouh Eswid, Bez El-Naka, Bez El-Anza, Romy Ahmer, Gharibi, Fayoumi and Romy Abiad, were evaluated for tolerance under saline conditions. Salinity stress was induced in three levels of 2.28, 3.75 and 5.20 ms (using NaCl at 1000, 2000 and 3000 ppm, respectively) comparing to 695 µs irrigation water as control. Results indicated that, the growth of all investigated local grape genotypes was adversely affected by salt treatments in a cultivar-dependent manner. Proposed salt-tolerance mechanisms including; controlling the growth rate, reducing damage resulting from oxidative stress associated with salinity, keep balanced hydric status, structural alterations allowing protection and regulation of ions uptake. It was observed that, Edkawy local grape cultivar is a promising salt-tolerant genotype. On the other hand, Romy Abiad and Gharibi genotypes were classified as the most salt-sensitive comparable with other tested local cultivars. Bez El-Anza genotype which maintained 100% survivability under severe salinity stress condition was characterized by a remarkable decline in vegetative growth accompanied with keeping more leaves with a marked reduction in leaf area and most measurements of certain anatomical features, slight Na uptake, but undisputed oxidative stress indicators and down-regulated expression folds of AREB2 transcription factor; a sugar accumulation regulatory related gene. Therefore, local genotypes of Egyptian table grapes can be considered a storehouse of germplasm that should be conserved and not threatened with extinction or complete loss because they are adapted to severe environmental conditions and harsh cultural managements. VL - 11 IS - 6 ER -
Deciduous Fruits Research Department, Biotechnology Central Laboratory, Horticulture Research Institute (HRI), Agricultural Research Center (ARC), Giza, Egypt
Medicinal and Aromatic Plants Research Department, Biotechnology Central Laboratory, Horticulture Research Institute (HRI), Agricultural Research Center (ARC), Giza, Egypt
Fruit Crops Handling Research Department, Chemical Analysis Central Laboratory, Horticulture Research Institute (HRI), Agricultural Research Center (ARC), Giza, Egypt
Viticulture Research Department, Horticulture Research Institute (HRI), Agricultural Research Center (ARC), Giza, Egypt
Fruit Breeding Department, Horticulture Research Institute (HRI), Agricultural Research Center (ARC), Giza, Egypt
Medicinal and Aromatic Plants Research Department, Biotechnology Central Laboratory, Horticulture Research Institute (HRI), Agricultural Research Center (ARC), Giza, Egypt
