Biosciences Laboratory, Sciences and Technologies Doctoral School, Joseph KI-ZERBO University, Ouagadougou, Burkina Faso
Natural Substances, Natural Product Technologies and Environment Laboratory, Institute for Research in Applied Sciences and Technologies, National Center for Scientific and Technological Research, Ouagadougou, Burkina Faso
Natural Substances, Natural Product Technologies and Environment Laboratory, Institute for Research in Applied Sciences and Technologies, National Center for Scientific and Technological Research, Ouagadougou, Burkina Faso
Rice is one of the most widely produced cereals in Burkina Faso. However, the fungi transmitted by rice seeds, Oryza sativa L, cause enormous production losses in rice fields. The aim of this study was to assess the efficacy of powdery formulations of essential oils against Magnaporthe oryzae in rice. Thus, rice seeds of the FKR64 variety were disinfected with 15% sodium hypochlorite for 10 minutes and soaked in the suspension of 104 conidia/ml for 24 hours. These contaminated rice seeds were coated into powdery formulations of essential oils of L. multiflora,C. schoenanthus and their combination at a dose of 10g/kg of seed. A contaminated control was used. The experimental set-up was a completely randomised block with four treatments in three replicates, one of which was an absolute control. These treatments were carried out on L. multiflora essential oil, C. schoenanthus essential oil and their combination at a proportion of 50%. The results showed that the essential oil of L. multiflora recorded the highest percentage emergence, average height and average number of tillers of the plants, with values of 97.33%, 47.67 cm and 7.89 tillers respectively. This same formulation recorded the lowest percentage of seedling melt and foliar incidence, at 1.33% and 11.67% respectively. These essential oil formulations can be recommended for seed treatment against Magnaporthe oryzae.
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Rice production in Burkina Faso faces a number of constraints, including fungal diseases, which account for 70% of crop diseases. Rice blast is the most dangerous of these diseases
[1]
Bouet A., Vales M., Amancho N. A., Kouassi N. K et Sorho F. 2012. Evolution de la résistance partielle à la pyriculariose foliaire selon l’âge, chez le riz de la sous-espèce japonica [Age-related changes in partial resistance to leaf blast of the japonica subspecies of rice]. Int. J. Biol. Chem. Sci. 6(1): 337-354.
. It can cause seedling melting and even leaf spot
[2]
Mahomed, T., Elisabeth, Z. P., Ignace, S., Romain, S. W., & Roger, N. H. C. (2022). Efficacité fongicide de trois biopesticides à base d’huiles essentielles sur les principaux champignons associés aux semences de niébé au Burkina Faso [Fungicidal efficacy of three essential oil biopesticides on the main fungi associated with cowpea seeds in Burkina Faso]. J. Soc. Ouest-Afr. Chim., 051, 38-44.
[2]
. Infection can reach the seeds before harvest via the panicle, or after harvest, during drying and storage. These affections are among the main causes of production quality deterioration and yield reduction
[3]
Gnacadja, C., Vieira-Dalode, G., Razanaboahirana, C., Azokpota, P., Soumanou, M. M., & Sie, M. (2018). Revue analytique des performances agronomiques, nutritionnelles et perspectives de valorisation du riz africain [Analytical review of the agronomic and nutritional performance of African rice and prospects for its development] (Oryza glaberrima). Journal of Applied Biosciences, 122(1), 12211.
. The use of chemical seed treatment products remains the main means of combating this disease, but their use causes problems to human and animal health and to the environment
[4]
Idrissi M., Aït D. N., Ouammi L., Rhalem N., Soulaymani A. et Soulaymani R. B. 2010. Intoxication aigüe par les pesticides : Données du Centre Anti Poison du Maroc (1989-2007). Toxicologie Maroc, [Acute pesticide poisoning: Data from the Moroccan Poison Control Center (1989-2007). Toxicology Morocco] 4(1), 5-7.
[4]
. An alternative control method that is respectful of health and the environment therefore needs to be considered. Research into the fungicidal potential of aromatic plants has enabled the development of essential oil formulations for field or post-harvest protection of rice seeds
[5]
Sawadogo I., Ki H., Ouédraogo A. R., Ouédraogo I., Nébié R. C. H. 2018. Efficacité en milieu paysan de trois bio-insecticides à base d’huiles essentielles en protection post récolte du maïs. SNA, spécial hors-série [Efficacy in farming environments of three essential oils for post-harvest protection of maize. SNA, special issue] n°4, 4, 243-250.
[5]
. The substances in these plants also have fungicidal properties
[6]
Koïta K., Neya B. F., Nana T. A., Sankara P. 2012. Activité antifongique d’extraits de plantes locales du Burkina Faso contre Puccinia arachidis Speg., agent pathogène de la rouille de l’arachide (Arachis hypogaea L.) [Antifungal activity of local plant extracts from Burkina Faso against Puccinia arachidis Speg, a pathogen of groundnut rust (Arachis hypogaea L.]. JABs, (57), 4142– 4150.
[7]
Bonzi S., Somda I., Sérémé P., Adam T. 2013. Efficacy of essential oils of Cymbopogon citratus (D. C.) Stapf, Lippia multiflora Moldenke and hot water in the control of seedborne fungi Phoma sorghina and their effects on Sorghum bicolor (L.) Moench seed germination and plants development in Burkina Faso. Net J Agric Sci, 1(4), 111-115.
[6, 7]
. The aim of this study is to assess the efficacy of essential oils of Lippia multiflora and Cymbopogon schoenanthus against Magnaporthe oryzae, in rice seed treatments.
2. Material and Methods
2.1. Biological Material
Rice seeds of the FKR64 rice variety were used. The strain of M. oryzae, pathogen of rice blast, was used.
2.2. Essential Oil Formulations
Three (03) Powder formulations were manufactured from the oils of L.multiflora, C. schoenanthus and their combination at the respective minimum inhibition doses of 0.6 µl/ml i.e. 30 µl/100g, 1.5 µl/ml i.e. 75 µl/100g and 0.3 µl/ml i.e. 15 µl/100g. Rice flour was used as a solid adjuvant in the manufacture of powder formulations of essential oils.
2.3. Preparation of the Inoculum
M.oryzae, isolated from infested leaves showing symptoms, was grown on PDA medium in the dark at 25°C. After 14 days of incubation, the conidium-laden surface was scraped with a sterile metal spatula. The mycelium was then suspended in distilled water and vortexed for one minute. The resulting suspension was filtered through muslin to separate the conidia from the mycelial fragments. After counting with a Malassez cell, the conidial suspensions were adjusted with sterile water to obtain final concentrations of104 conidia/ml.
2.4. Seed Treatment Procedure
Rice seeds were disinfected with 15% sodium hypochlorite for 10 minutes
[8]
Bahous M., Ouazzani Touhami A., Benkirane R. et Douira A. 2010. Influence de l’âge de la plante sur la réceptivité des feuilles du riz à la pyriculariose, l’helminthosporiose et la curvulariose. Revue Marocaine de Protection des Plantes, [Influence of plant age on the susceptibility of rice leaves to blast, helminthosporiosis and curvulariosis. Revue Marocaine de Protection des Plantes] N° 1: 73-84.
[8]
. followed by a sanitary assessment to ensure the absence of pathogens. These seeds were soaked in the suspension of 104 conidia/mlfor 24 hours. Control seeds were transferred to Erlenmeyer flasks containing distilled water for 24 hours. The inoculated seeds and the control seeds, dried in a laboratory hood for 24 hours, were coated in powdered formulations of essential oils and then sown in pots.
2.5. Preparing the Potting Soil
The potting mix consisted of a mixture of organic fertiliser and soil in proportions of one third (1/3) and two thirds (2/3) respectively. The potting soil is collected at the Farakoba station to a depth of 20 cm then sterilised in an autoclave at 121°C for 45 minutes.
2.6. Experimental Set-up
The experimental set-up was a completely randomised block with four treatments in three replicates, one of which was an absolute control (T0). These were treatments with the essential oil of L. multiflora (T1), with C. schoenanthus (T2) and with their combination at 50% proportion (T3).
2.7. Parameters Measured
The percentage of seedling emergence was assessed on the 14th day after sowing, the percentage of seedling melt on the 28th day after sowing, the percentage of plants with leaf spots, the number of tillers and the height of the plants on the 42nd day after sowing.
2.8. Statistical Analysis
Analysis of variance (ANOVA) was performed using XLSTAT software. Means were compared using the Fisher test at the 5% probability level.
3. Results
Table 1 shows the percentage of seedling emergence, seedling melt, foliar incidence, plant height and average number of tillers per plant according to the different essential oil formulations. For each parameter assessed, the analysis of variance showed a very highly significant difference between treatments at the 5% probability threshold using Fisher's test. The results showed that treatment T1 recorded the highest percentage of seedling emergence, plant height and number of tillers, with 97.33%, 47.67 cm and 7.89 tillers/plant respectively. The results showed that treatment T0 recorded the highest values for seedling melt and leaf incidence with 7.57% and 36.45% respectively. Treatment T0 recorded the lowest values for percentage emergence, plant height and number of tillers with 86.96%, 27.33 cm and 3.67 tillers/plant respectively. Treatment T1 recorded the lowest percentages of seedling melt and foliar incidence with 1.33% and 11.67% respectively.
Table 1. Parameters assessed.
Treatments
Emergence percentage
Percentage of seedling melt
Foliar incidence
T0
86,96c
7,57a
36,45a
T1
97,33a
1,33d
11,67c
T2
92,21b
3,33b
24,33b
T3
92,54b
2,67c
23,67b
Prob
< 0,001
< 0,001
< 0,001
Signification
THS
THS
THS
Legend: Values in the same column with the same letter are not statistically different at the 5% probability level according to the Fisher test.
Graphs marked with the same letter are not statistically different at the 5% probability level according to the Fisher test.
4. Discussion
The application of powdery formulations of L. multiflora and C. schoenanthus essential oils and their combination to seeds artificially contaminated by M. oryzae demonstrated their antifungal effect. They reduced seed infection by improving the emergence rate and reducing the rate of seedling melt and foliar incidence compared to the control. The effectiveness of essential oils can be explained by their content of major elements, their antifungal properties and their chemical compositions, which enable them to stop or slow down the development of the fungus, or by the synergistic or additive effect of the chemical compounds. This effectiveness of essential oils has been reported by
[9]
Ambindei W. A., Ngouné L. T., Sameza M. L., Sonwa E. T., Nguimatsia F., Dongmo P. M. J. 2014. Antifungal Activities against some Aspergillus species of the Essential oils of Canarium schweinfurthii and Aucoumea klaineana growing in Cameroon. Int. J. Curr. Microbiol. App. Sci. 3(5), 691-701.
[9]
.
Similar results have been obtained by
[2]
Mahomed, T., Elisabeth, Z. P., Ignace, S., Romain, S. W., & Roger, N. H. C. (2022). Efficacité fongicide de trois biopesticides à base d’huiles essentielles sur les principaux champignons associés aux semences de niébé au Burkina Faso [Fungicidal efficacy of three essential oil biopesticides on the main fungi associated with cowpea seeds in Burkina Faso]. J. Soc. Ouest-Afr. Chim., 051, 38-44.
[2]
who demonstrated that the fungicidal effect of three formulations based on essential oils of L. multiflora, C. schoenanthus and O. americanum in the protection of cowpea seeds. In addition, the work of
[10]
Adjou E. S. et Soumanou M. M. 2013. Efficacité des extraits de plantes dans la lutte contre les moisissures toxinogènes isolées de l’arachide en post-récolte au Bénin [Efficacy of plant extracts in the control of toxigenic moulds isolated from post-harvest groundnuts in Benin]. Journal of Applied Biosciences. 70, 5555– 5566.
on fungi isolated from groundnuts in Benin showed the antifungal activity of essential oils and aqueous extracts of O. americanum on several toxigenic strains of the genus Aspergillus and Fusarium. The work of
[11]
Zida P. E, Sérémé P, Leth V, Sankara P, Somda I, Néya A. 2008. Importance of seed-borne fungi on sorghum and pearl millet in Burkina Faso and their control using plant extracts. Pakistan Journal of Biological Sciences. 11, 321-331.
demonstrated the efficacy of C. schoenanthus essential oils against sorghum and millet seed-borne fungi. The work of
[12]
Gbogbo K. A., Komlan B., Kouassi A., Mireille P. D., Messanvi G., Philippe B., Koffi A. 2006. Activité antifongique des huiles essentielles de Ocimum basilicum L. (Lamiaceae) et Cymbopogon schoenanthus (L.) Spreng. (Poaceae) sur des micromycètes influençant la germination du maïs et du niébé [Antifungal activity of essential oils of Ocimum basilicum L. (Lamiaceae) and Cymbopogon schoenanthus (L.) Spreng (Poaceae) on micromycetes influencing the germination of maize and cowpeas]. Acta Bot. Gallica, 153(1), 115-124.
carried out on the seeds of some cereals such as maize in the presence of phytopathogenic and toxinogenic fungi in Togo showed that the essential oils of O. basilicum and C. schoenanthus have antifungal activity by improving the germination rate of the seeds of these two crops.
The results showed that the essential oils and their combination had a remarkable effect on the morphological parameters of the rice plants by improving the plant height and number of tillers. However, the essential oil of L. multiflora had a more marked effect in improving the number of tillers and plant height. This can be explained by the fact that essential oil formulations, depending on their chemical composition, do not regulate M. oryzae in the same way. Similar work has been reported by
[13]
Kone, Y., Sangare, M. B., Dagno, K., Niangaly, A., Doumbia, S., Amadou, H., & Kourouma, M. (2023). Efficacy of several species of fungi bioagents and fungicides against Magnaporthe oryzae in vitro. International Journal of Biological and Chemical Sciences, 17(3), 935‑949.
Kobenan K. C., Kouakou B. J., Bini K. K. N., Kouakou M., Dick A. E. et Ochou O. G. 2019. Effets des Huiles Essentielles de Ocimum gratissimum L. et de Cymbopogon citratus Stapf sur les Paramètres de Croissance et de Production du Cotonnier en Côte d’Ivoire [Effects of Essential Oils of Ocimum gratissimum L. and Cymbopogon citratus Stapf on Growth and Production Parameters of Cotton in Côte d'Ivoire]. European Journal of Scientific Research, pp. 21-35.
[15]
Soro S., Ouattara D., Zirihi G. N., Kanko C., N’guessan E. K., Kone D., Kouadio J. Y., Ake S. 2010. Effet inhibiteur in vitro et in vivo de l’extrait de poudre et de l’huile essentielle de Xylopia aethiopica (Dunal) A. Rich. (Annonaceae) sur Fusarium oxysporum f. sp radicis-lycopersici (Forl), champignon parasite des cultures de tomate. [In vitro and in vivo inhibitory effect of powder extract and essential oil of Xylopia aethiopica (Dunal) A. Rich. (Annonaceae) on Fusarium oxysporum f. sp radicis-lycopersici (Forl), a fungal parasite of tomato crops] European Journal of Scientific Research 39(2): 279-288.
[14, 15]
respectively showed that the application of essential oils of O. gratissimum and C. citratus to cotton resulted in good fibre yields, while the application of essential oil of X.Aethiopica against F. oxysporum produced high leaf and root biomass compared with the control.
5. Conclusion
This study demonstrated the efficacy of essential oils as seed treatments against M. oryzae. All the essential oil formulations of L. multiflora, C. schoenanthus and their combination showed an antifungal effect and improved the morphological parameters of the rice plants. However, the essential oil of L. multiflora had a more marked effect in antifungal activity and in improving morphological parameters. These essential oil formulations can be recommended for the treatment and conservation of seeds against fungi.
Bouet A., Vales M., Amancho N. A., Kouassi N. K et Sorho F. 2012. Evolution de la résistance partielle à la pyriculariose foliaire selon l’âge, chez le riz de la sous-espèce japonica [Age-related changes in partial resistance to leaf blast of the japonica subspecies of rice]. Int. J. Biol. Chem. Sci. 6(1): 337-354.
Mahomed, T., Elisabeth, Z. P., Ignace, S., Romain, S. W., & Roger, N. H. C. (2022). Efficacité fongicide de trois biopesticides à base d’huiles essentielles sur les principaux champignons associés aux semences de niébé au Burkina Faso [Fungicidal efficacy of three essential oil biopesticides on the main fungi associated with cowpea seeds in Burkina Faso]. J. Soc. Ouest-Afr. Chim., 051, 38-44.
[3]
Gnacadja, C., Vieira-Dalode, G., Razanaboahirana, C., Azokpota, P., Soumanou, M. M., & Sie, M. (2018). Revue analytique des performances agronomiques, nutritionnelles et perspectives de valorisation du riz africain [Analytical review of the agronomic and nutritional performance of African rice and prospects for its development] (Oryza glaberrima). Journal of Applied Biosciences, 122(1), 12211.
Idrissi M., Aït D. N., Ouammi L., Rhalem N., Soulaymani A. et Soulaymani R. B. 2010. Intoxication aigüe par les pesticides : Données du Centre Anti Poison du Maroc (1989-2007). Toxicologie Maroc, [Acute pesticide poisoning: Data from the Moroccan Poison Control Center (1989-2007). Toxicology Morocco] 4(1), 5-7.
[5]
Sawadogo I., Ki H., Ouédraogo A. R., Ouédraogo I., Nébié R. C. H. 2018. Efficacité en milieu paysan de trois bio-insecticides à base d’huiles essentielles en protection post récolte du maïs. SNA, spécial hors-série [Efficacy in farming environments of three essential oils for post-harvest protection of maize. SNA, special issue] n°4, 4, 243-250.
[6]
Koïta K., Neya B. F., Nana T. A., Sankara P. 2012. Activité antifongique d’extraits de plantes locales du Burkina Faso contre Puccinia arachidis Speg., agent pathogène de la rouille de l’arachide (Arachis hypogaea L.) [Antifungal activity of local plant extracts from Burkina Faso against Puccinia arachidis Speg, a pathogen of groundnut rust (Arachis hypogaea L.]. JABs, (57), 4142– 4150.
[7]
Bonzi S., Somda I., Sérémé P., Adam T. 2013. Efficacy of essential oils of Cymbopogon citratus (D. C.) Stapf, Lippia multiflora Moldenke and hot water in the control of seedborne fungi Phoma sorghina and their effects on Sorghum bicolor (L.) Moench seed germination and plants development in Burkina Faso. Net J Agric Sci, 1(4), 111-115.
[8]
Bahous M., Ouazzani Touhami A., Benkirane R. et Douira A. 2010. Influence de l’âge de la plante sur la réceptivité des feuilles du riz à la pyriculariose, l’helminthosporiose et la curvulariose. Revue Marocaine de Protection des Plantes, [Influence of plant age on the susceptibility of rice leaves to blast, helminthosporiosis and curvulariosis. Revue Marocaine de Protection des Plantes] N° 1: 73-84.
[9]
Ambindei W. A., Ngouné L. T., Sameza M. L., Sonwa E. T., Nguimatsia F., Dongmo P. M. J. 2014. Antifungal Activities against some Aspergillus species of the Essential oils of Canarium schweinfurthii and Aucoumea klaineana growing in Cameroon. Int. J. Curr. Microbiol. App. Sci. 3(5), 691-701.
[10]
Adjou E. S. et Soumanou M. M. 2013. Efficacité des extraits de plantes dans la lutte contre les moisissures toxinogènes isolées de l’arachide en post-récolte au Bénin [Efficacy of plant extracts in the control of toxigenic moulds isolated from post-harvest groundnuts in Benin]. Journal of Applied Biosciences. 70, 5555– 5566.
Zida P. E, Sérémé P, Leth V, Sankara P, Somda I, Néya A. 2008. Importance of seed-borne fungi on sorghum and pearl millet in Burkina Faso and their control using plant extracts. Pakistan Journal of Biological Sciences. 11, 321-331.
Gbogbo K. A., Komlan B., Kouassi A., Mireille P. D., Messanvi G., Philippe B., Koffi A. 2006. Activité antifongique des huiles essentielles de Ocimum basilicum L. (Lamiaceae) et Cymbopogon schoenanthus (L.) Spreng. (Poaceae) sur des micromycètes influençant la germination du maïs et du niébé [Antifungal activity of essential oils of Ocimum basilicum L. (Lamiaceae) and Cymbopogon schoenanthus (L.) Spreng (Poaceae) on micromycetes influencing the germination of maize and cowpeas]. Acta Bot. Gallica, 153(1), 115-124.
Kone, Y., Sangare, M. B., Dagno, K., Niangaly, A., Doumbia, S., Amadou, H., & Kourouma, M. (2023). Efficacy of several species of fungi bioagents and fungicides against Magnaporthe oryzae in vitro. International Journal of Biological and Chemical Sciences, 17(3), 935‑949.
Kobenan K. C., Kouakou B. J., Bini K. K. N., Kouakou M., Dick A. E. et Ochou O. G. 2019. Effets des Huiles Essentielles de Ocimum gratissimum L. et de Cymbopogon citratus Stapf sur les Paramètres de Croissance et de Production du Cotonnier en Côte d’Ivoire [Effects of Essential Oils of Ocimum gratissimum L. and Cymbopogon citratus Stapf on Growth and Production Parameters of Cotton in Côte d'Ivoire]. European Journal of Scientific Research, pp. 21-35.
[15]
Soro S., Ouattara D., Zirihi G. N., Kanko C., N’guessan E. K., Kone D., Kouadio J. Y., Ake S. 2010. Effet inhibiteur in vitro et in vivo de l’extrait de poudre et de l’huile essentielle de Xylopia aethiopica (Dunal) A. Rich. (Annonaceae) sur Fusarium oxysporum f. sp radicis-lycopersici (Forl), champignon parasite des cultures de tomate. [In vitro and in vivo inhibitory effect of powder extract and essential oil of Xylopia aethiopica (Dunal) A. Rich. (Annonaceae) on Fusarium oxysporum f. sp radicis-lycopersici (Forl), a fungal parasite of tomato crops] European Journal of Scientific Research 39(2): 279-288.
Souleymane, O., Itolou, K. A., Sylvain, Z., Abdoulaye, S., Kadidia, K. (2024). Efficacy of Essential Oil Formulations in Rice Seed Treatment Against Magnaporthe oryzae B.C Couch, a Rice Blast Pathogen. American Journal of BioScience, 12(2), 75-79. https://doi.org/10.11648/j.ajbio.20241202.15
Souleymane O, Itolou KA, Sylvain Z, Abdoulaye S, Kadidia K. Efficacy of Essential Oil Formulations in Rice Seed Treatment Against Magnaporthe oryzae B.C Couch, a Rice Blast Pathogen. Am J BioScience. 2024;12(2):75-79. doi: 10.11648/j.ajbio.20241202.15
@article{10.11648/j.ajbio.20241202.15,
author = {Ouattara Souleymane and Kassankogno Abalo Itolou and Zougrana Sylvain and Sérémé Abdoulaye and Koïta Kadidia},
title = {Efficacy of Essential Oil Formulations in Rice Seed Treatment Against Magnaporthe oryzae B.C Couch, a Rice Blast Pathogen
},
journal = {American Journal of BioScience},
volume = {12},
number = {2},
pages = {75-79},
doi = {10.11648/j.ajbio.20241202.15},
url = {https://doi.org/10.11648/j.ajbio.20241202.15},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20241202.15},
abstract = {Rice is one of the most widely produced cereals in Burkina Faso. However, the fungi transmitted by rice seeds, Oryza sativa L, cause enormous production losses in rice fields. The aim of this study was to assess the efficacy of powdery formulations of essential oils against Magnaporthe oryzae in rice. Thus, rice seeds of the FKR64 variety were disinfected with 15% sodium hypochlorite for 10 minutes and soaked in the suspension of 104 conidia/ml for 24 hours. These contaminated rice seeds were coated into powdery formulations of essential oils of L. multiflora, C. schoenanthus and their combination at a dose of 10g/kg of seed. A contaminated control was used. The experimental set-up was a completely randomised block with four treatments in three replicates, one of which was an absolute control. These treatments were carried out on L. multiflora essential oil, C. schoenanthus essential oil and their combination at a proportion of 50%. The results showed that the essential oil of L. multiflora recorded the highest percentage emergence, average height and average number of tillers of the plants, with values of 97.33%, 47.67 cm and 7.89 tillers respectively. This same formulation recorded the lowest percentage of seedling melt and foliar incidence, at 1.33% and 11.67% respectively. These essential oil formulations can be recommended for seed treatment against Magnaporthe oryzae.
},
year = {2024}
}
TY - JOUR
T1 - Efficacy of Essential Oil Formulations in Rice Seed Treatment Against Magnaporthe oryzae B.C Couch, a Rice Blast Pathogen
AU - Ouattara Souleymane
AU - Kassankogno Abalo Itolou
AU - Zougrana Sylvain
AU - Sérémé Abdoulaye
AU - Koïta Kadidia
Y1 - 2024/04/29
PY - 2024
N1 - https://doi.org/10.11648/j.ajbio.20241202.15
DO - 10.11648/j.ajbio.20241202.15
T2 - American Journal of BioScience
JF - American Journal of BioScience
JO - American Journal of BioScience
SP - 75
EP - 79
PB - Science Publishing Group
SN - 2330-0167
UR - https://doi.org/10.11648/j.ajbio.20241202.15
AB - Rice is one of the most widely produced cereals in Burkina Faso. However, the fungi transmitted by rice seeds, Oryza sativa L, cause enormous production losses in rice fields. The aim of this study was to assess the efficacy of powdery formulations of essential oils against Magnaporthe oryzae in rice. Thus, rice seeds of the FKR64 variety were disinfected with 15% sodium hypochlorite for 10 minutes and soaked in the suspension of 104 conidia/ml for 24 hours. These contaminated rice seeds were coated into powdery formulations of essential oils of L. multiflora, C. schoenanthus and their combination at a dose of 10g/kg of seed. A contaminated control was used. The experimental set-up was a completely randomised block with four treatments in three replicates, one of which was an absolute control. These treatments were carried out on L. multiflora essential oil, C. schoenanthus essential oil and their combination at a proportion of 50%. The results showed that the essential oil of L. multiflora recorded the highest percentage emergence, average height and average number of tillers of the plants, with values of 97.33%, 47.67 cm and 7.89 tillers respectively. This same formulation recorded the lowest percentage of seedling melt and foliar incidence, at 1.33% and 11.67% respectively. These essential oil formulations can be recommended for seed treatment against Magnaporthe oryzae.
VL - 12
IS - 2
ER -
Biosciences Laboratory, Sciences and Technologies Doctoral School, Joseph KI-ZERBO University, Ouagadougou, Burkina Faso; Natural Substances, Natural Product Technologies and Environment Laboratory, Institute for Research in Applied Sciences and Technologies, National Center for Scientific and Technological Research, Ouagadougou, Burkina Faso
Natural Substances, Natural Product Technologies and Environment Laboratory, Institute for Research in Applied Sciences and Technologies, National Center for Scientific and Technological Research, Ouagadougou, Burkina Faso
Souleymane, O., Itolou, K. A., Sylvain, Z., Abdoulaye, S., Kadidia, K. (2024). Efficacy of Essential Oil Formulations in Rice Seed Treatment Against Magnaporthe oryzae B.C Couch, a Rice Blast Pathogen. American Journal of BioScience, 12(2), 75-79. https://doi.org/10.11648/j.ajbio.20241202.15
Souleymane O, Itolou KA, Sylvain Z, Abdoulaye S, Kadidia K. Efficacy of Essential Oil Formulations in Rice Seed Treatment Against Magnaporthe oryzae B.C Couch, a Rice Blast Pathogen. Am J BioScience. 2024;12(2):75-79. doi: 10.11648/j.ajbio.20241202.15
@article{10.11648/j.ajbio.20241202.15,
author = {Ouattara Souleymane and Kassankogno Abalo Itolou and Zougrana Sylvain and Sérémé Abdoulaye and Koïta Kadidia},
title = {Efficacy of Essential Oil Formulations in Rice Seed Treatment Against Magnaporthe oryzae B.C Couch, a Rice Blast Pathogen
},
journal = {American Journal of BioScience},
volume = {12},
number = {2},
pages = {75-79},
doi = {10.11648/j.ajbio.20241202.15},
url = {https://doi.org/10.11648/j.ajbio.20241202.15},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20241202.15},
abstract = {Rice is one of the most widely produced cereals in Burkina Faso. However, the fungi transmitted by rice seeds, Oryza sativa L, cause enormous production losses in rice fields. The aim of this study was to assess the efficacy of powdery formulations of essential oils against Magnaporthe oryzae in rice. Thus, rice seeds of the FKR64 variety were disinfected with 15% sodium hypochlorite for 10 minutes and soaked in the suspension of 104 conidia/ml for 24 hours. These contaminated rice seeds were coated into powdery formulations of essential oils of L. multiflora, C. schoenanthus and their combination at a dose of 10g/kg of seed. A contaminated control was used. The experimental set-up was a completely randomised block with four treatments in three replicates, one of which was an absolute control. These treatments were carried out on L. multiflora essential oil, C. schoenanthus essential oil and their combination at a proportion of 50%. The results showed that the essential oil of L. multiflora recorded the highest percentage emergence, average height and average number of tillers of the plants, with values of 97.33%, 47.67 cm and 7.89 tillers respectively. This same formulation recorded the lowest percentage of seedling melt and foliar incidence, at 1.33% and 11.67% respectively. These essential oil formulations can be recommended for seed treatment against Magnaporthe oryzae.
},
year = {2024}
}
TY - JOUR
T1 - Efficacy of Essential Oil Formulations in Rice Seed Treatment Against Magnaporthe oryzae B.C Couch, a Rice Blast Pathogen
AU - Ouattara Souleymane
AU - Kassankogno Abalo Itolou
AU - Zougrana Sylvain
AU - Sérémé Abdoulaye
AU - Koïta Kadidia
Y1 - 2024/04/29
PY - 2024
N1 - https://doi.org/10.11648/j.ajbio.20241202.15
DO - 10.11648/j.ajbio.20241202.15
T2 - American Journal of BioScience
JF - American Journal of BioScience
JO - American Journal of BioScience
SP - 75
EP - 79
PB - Science Publishing Group
SN - 2330-0167
UR - https://doi.org/10.11648/j.ajbio.20241202.15
AB - Rice is one of the most widely produced cereals in Burkina Faso. However, the fungi transmitted by rice seeds, Oryza sativa L, cause enormous production losses in rice fields. The aim of this study was to assess the efficacy of powdery formulations of essential oils against Magnaporthe oryzae in rice. Thus, rice seeds of the FKR64 variety were disinfected with 15% sodium hypochlorite for 10 minutes and soaked in the suspension of 104 conidia/ml for 24 hours. These contaminated rice seeds were coated into powdery formulations of essential oils of L. multiflora, C. schoenanthus and their combination at a dose of 10g/kg of seed. A contaminated control was used. The experimental set-up was a completely randomised block with four treatments in three replicates, one of which was an absolute control. These treatments were carried out on L. multiflora essential oil, C. schoenanthus essential oil and their combination at a proportion of 50%. The results showed that the essential oil of L. multiflora recorded the highest percentage emergence, average height and average number of tillers of the plants, with values of 97.33%, 47.67 cm and 7.89 tillers respectively. This same formulation recorded the lowest percentage of seedling melt and foliar incidence, at 1.33% and 11.67% respectively. These essential oil formulations can be recommended for seed treatment against Magnaporthe oryzae.
VL - 12
IS - 2
ER -
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