Preliminary Evaluation of an Autologous Dendritic Cell Vaccine Using Nanoparticle Technology for the Treatment of Canine Malignant Melanoma

Meaghan Veronica Eren; Julianne Hwang; Janean Fidel; Rance Sellon; Cleverson de Souza

doi:doi:10.11648/j.ajbls.20210901.21

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Preliminary Evaluation of an Autologous Dendritic Cell Vaccine Using Nanoparticle Technology for the Treatment of Canine Malignant Melanoma

Meaghan Veronica Eren, Julianne Hwang, Janean Fidel, Rance Sellon, Cleverson de Souza

Published in American Journal of Biomedical and Life Sciences (Volume 9, Issue 1)

Received: 3 February 2021 Accepted: 10 February 2021 Published: 23 February 2021

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Abstract

Canine malignant melanoma is an aggressive neoplasm that carries a poor prognosis due to its minimal responsiveness to traditional therapy protocols, particularly if the oral cavity, mucocutaneous junctions, or subungual sites are involved. This proof-of-concept study evaluated a prototype autologous dendritic cell vaccine using poly-lactic-co-glycolic (PLGA) nanoparticles containing antigens from patient-derived whole tumor lysate and the adjuvant monophosphoryl lipid A in five canines with stages III-IV malignant melanoma. Nanoparticle constructs biochemical characterization; encapsulation efficiency and kinetic release studies were determined. Our results showed that tumor antigens were successfully incorporated in the PLGA/monophosphoryl lipid A nanoparticle constructs. Additional in vitro experiments showed that the PLGA/monophosphoryl lipid A nanoparticle constructs effectively activated autologous dendritic cells, and generated a greater than twofold increase in the release of the pro-immune cytokine IFN-γ. No significant adverse effects were observed in any of the patients following intradermal vaccination, and flow cytometry of whole blood revealed increased CD4:CD8 T lymphocyte ratios by the completion of the study. These results suggest that a dendritic cell vaccine utilizing PLGA/monophosphoryl lipid A nanoparticle technology could potentially initiate an adaptive immune response and is safe to administer to canine patients. Further in vivo studies with a larger cohort of patients are warranted.

Published in	American Journal of Biomedical and Life Sciences (Volume 9, Issue 1)
DOI	10.11648/j.ajbls.20210901.21
Page(s)	84-96
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Melanoma, Nanoparticles, Dendritic Cells, Adaptive Immunity, Cancer Vaccines

References

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APA Style

Meaghan Veronica Eren, Julianne Hwang, Janean Fidel, Rance Sellon, Cleverson de Souza. (2021). Preliminary Evaluation of an Autologous Dendritic Cell Vaccine Using Nanoparticle Technology for the Treatment of Canine Malignant Melanoma. American Journal of Biomedical and Life Sciences, 9(1), 84-96. https://doi.org/10.11648/j.ajbls.20210901.21

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ACS Style

Meaghan Veronica Eren; Julianne Hwang; Janean Fidel; Rance Sellon; Cleverson de Souza. Preliminary Evaluation of an Autologous Dendritic Cell Vaccine Using Nanoparticle Technology for the Treatment of Canine Malignant Melanoma. Am. J. Biomed. Life Sci. 2021, 9(1), 84-96. doi: 10.11648/j.ajbls.20210901.21

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AMA Style

Meaghan Veronica Eren, Julianne Hwang, Janean Fidel, Rance Sellon, Cleverson de Souza. Preliminary Evaluation of an Autologous Dendritic Cell Vaccine Using Nanoparticle Technology for the Treatment of Canine Malignant Melanoma. Am J Biomed Life Sci. 2021;9(1):84-96. doi: 10.11648/j.ajbls.20210901.21

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@article{10.11648/j.ajbls.20210901.21,
  author = {Meaghan Veronica Eren and Julianne Hwang and Janean Fidel and Rance Sellon and Cleverson de Souza},
  title = {Preliminary Evaluation of an Autologous Dendritic Cell Vaccine Using Nanoparticle Technology for the Treatment of Canine Malignant Melanoma},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {9},
  number = {1},
  pages = {84-96},
  doi = {10.11648/j.ajbls.20210901.21},
  url = {https://doi.org/10.11648/j.ajbls.20210901.21},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20210901.21},
  abstract = {Canine malignant melanoma is an aggressive neoplasm that carries a poor prognosis due to its minimal responsiveness to traditional therapy protocols, particularly if the oral cavity, mucocutaneous junctions, or subungual sites are involved. This proof-of-concept study evaluated a prototype autologous dendritic cell vaccine using poly-lactic-co-glycolic (PLGA) nanoparticles containing antigens from patient-derived whole tumor lysate and the adjuvant monophosphoryl lipid A in five canines with stages III-IV malignant melanoma. Nanoparticle constructs biochemical characterization; encapsulation efficiency and kinetic release studies were determined. Our results showed that tumor antigens were successfully incorporated in the PLGA/monophosphoryl lipid A nanoparticle constructs. Additional in vitro experiments showed that the PLGA/monophosphoryl lipid A nanoparticle constructs effectively activated autologous dendritic cells, and generated a greater than twofold increase in the release of the pro-immune cytokine IFN-γ. No significant adverse effects were observed in any of the patients following intradermal vaccination, and flow cytometry of whole blood revealed increased CD4:CD8 T lymphocyte ratios by the completion of the study. These results suggest that a dendritic cell vaccine utilizing PLGA/monophosphoryl lipid A nanoparticle technology could potentially initiate an adaptive immune response and is safe to administer to canine patients. Further in vivo studies with a larger cohort of patients are warranted.},
 year = {2021}
}

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TY  - JOUR
T1  - Preliminary Evaluation of an Autologous Dendritic Cell Vaccine Using Nanoparticle Technology for the Treatment of Canine Malignant Melanoma
AU  - Meaghan Veronica Eren
AU  - Julianne Hwang
AU  - Janean Fidel
AU  - Rance Sellon
AU  - Cleverson de Souza
Y1  - 2021/02/23
PY  - 2021
N1  - https://doi.org/10.11648/j.ajbls.20210901.21
DO  - 10.11648/j.ajbls.20210901.21
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 84
EP  - 96
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20210901.21
AB  - Canine malignant melanoma is an aggressive neoplasm that carries a poor prognosis due to its minimal responsiveness to traditional therapy protocols, particularly if the oral cavity, mucocutaneous junctions, or subungual sites are involved. This proof-of-concept study evaluated a prototype autologous dendritic cell vaccine using poly-lactic-co-glycolic (PLGA) nanoparticles containing antigens from patient-derived whole tumor lysate and the adjuvant monophosphoryl lipid A in five canines with stages III-IV malignant melanoma. Nanoparticle constructs biochemical characterization; encapsulation efficiency and kinetic release studies were determined. Our results showed that tumor antigens were successfully incorporated in the PLGA/monophosphoryl lipid A nanoparticle constructs. Additional in vitro experiments showed that the PLGA/monophosphoryl lipid A nanoparticle constructs effectively activated autologous dendritic cells, and generated a greater than twofold increase in the release of the pro-immune cytokine IFN-γ. No significant adverse effects were observed in any of the patients following intradermal vaccination, and flow cytometry of whole blood revealed increased CD4:CD8 T lymphocyte ratios by the completion of the study. These results suggest that a dendritic cell vaccine utilizing PLGA/monophosphoryl lipid A nanoparticle technology could potentially initiate an adaptive immune response and is safe to administer to canine patients. Further in vivo studies with a larger cohort of patients are warranted.
VL  - 9
IS  - 1
ER  -

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Author Information

Meaghan Veronica Eren

Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, Washington, USA
Julianne Hwang

Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, Washington, USA
Janean Fidel

Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, Washington, USA
Rance Sellon

Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, Washington, USA
Cleverson de Souza

Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, Washington, USA

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Meaghan Veronica Eren, Julianne Hwang, Janean Fidel, Rance Sellon, Cleverson de Souza. (2021). Preliminary Evaluation of an Autologous Dendritic Cell Vaccine Using Nanoparticle Technology for the Treatment of Canine Malignant Melanoma. American Journal of Biomedical and Life Sciences, 9(1), 84-96. https://doi.org/10.11648/j.ajbls.20210901.21

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ACS Style

Meaghan Veronica Eren; Julianne Hwang; Janean Fidel; Rance Sellon; Cleverson de Souza. Preliminary Evaluation of an Autologous Dendritic Cell Vaccine Using Nanoparticle Technology for the Treatment of Canine Malignant Melanoma. Am. J. Biomed. Life Sci. 2021, 9(1), 84-96. doi: 10.11648/j.ajbls.20210901.21

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AMA Style

Meaghan Veronica Eren, Julianne Hwang, Janean Fidel, Rance Sellon, Cleverson de Souza. Preliminary Evaluation of an Autologous Dendritic Cell Vaccine Using Nanoparticle Technology for the Treatment of Canine Malignant Melanoma. Am J Biomed Life Sci. 2021;9(1):84-96. doi: 10.11648/j.ajbls.20210901.21

Copy | Download

@article{10.11648/j.ajbls.20210901.21,
  author = {Meaghan Veronica Eren and Julianne Hwang and Janean Fidel and Rance Sellon and Cleverson de Souza},
  title = {Preliminary Evaluation of an Autologous Dendritic Cell Vaccine Using Nanoparticle Technology for the Treatment of Canine Malignant Melanoma},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {9},
  number = {1},
  pages = {84-96},
  doi = {10.11648/j.ajbls.20210901.21},
  url = {https://doi.org/10.11648/j.ajbls.20210901.21},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20210901.21},
  abstract = {Canine malignant melanoma is an aggressive neoplasm that carries a poor prognosis due to its minimal responsiveness to traditional therapy protocols, particularly if the oral cavity, mucocutaneous junctions, or subungual sites are involved. This proof-of-concept study evaluated a prototype autologous dendritic cell vaccine using poly-lactic-co-glycolic (PLGA) nanoparticles containing antigens from patient-derived whole tumor lysate and the adjuvant monophosphoryl lipid A in five canines with stages III-IV malignant melanoma. Nanoparticle constructs biochemical characterization; encapsulation efficiency and kinetic release studies were determined. Our results showed that tumor antigens were successfully incorporated in the PLGA/monophosphoryl lipid A nanoparticle constructs. Additional in vitro experiments showed that the PLGA/monophosphoryl lipid A nanoparticle constructs effectively activated autologous dendritic cells, and generated a greater than twofold increase in the release of the pro-immune cytokine IFN-γ. No significant adverse effects were observed in any of the patients following intradermal vaccination, and flow cytometry of whole blood revealed increased CD4:CD8 T lymphocyte ratios by the completion of the study. These results suggest that a dendritic cell vaccine utilizing PLGA/monophosphoryl lipid A nanoparticle technology could potentially initiate an adaptive immune response and is safe to administer to canine patients. Further in vivo studies with a larger cohort of patients are warranted.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Preliminary Evaluation of an Autologous Dendritic Cell Vaccine Using Nanoparticle Technology for the Treatment of Canine Malignant Melanoma
AU  - Meaghan Veronica Eren
AU  - Julianne Hwang
AU  - Janean Fidel
AU  - Rance Sellon
AU  - Cleverson de Souza
Y1  - 2021/02/23
PY  - 2021
N1  - https://doi.org/10.11648/j.ajbls.20210901.21
DO  - 10.11648/j.ajbls.20210901.21
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 84
EP  - 96
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20210901.21
AB  - Canine malignant melanoma is an aggressive neoplasm that carries a poor prognosis due to its minimal responsiveness to traditional therapy protocols, particularly if the oral cavity, mucocutaneous junctions, or subungual sites are involved. This proof-of-concept study evaluated a prototype autologous dendritic cell vaccine using poly-lactic-co-glycolic (PLGA) nanoparticles containing antigens from patient-derived whole tumor lysate and the adjuvant monophosphoryl lipid A in five canines with stages III-IV malignant melanoma. Nanoparticle constructs biochemical characterization; encapsulation efficiency and kinetic release studies were determined. Our results showed that tumor antigens were successfully incorporated in the PLGA/monophosphoryl lipid A nanoparticle constructs. Additional in vitro experiments showed that the PLGA/monophosphoryl lipid A nanoparticle constructs effectively activated autologous dendritic cells, and generated a greater than twofold increase in the release of the pro-immune cytokine IFN-γ. No significant adverse effects were observed in any of the patients following intradermal vaccination, and flow cytometry of whole blood revealed increased CD4:CD8 T lymphocyte ratios by the completion of the study. These results suggest that a dendritic cell vaccine utilizing PLGA/monophosphoryl lipid A nanoparticle technology could potentially initiate an adaptive immune response and is safe to administer to canine patients. Further in vivo studies with a larger cohort of patients are warranted.
VL  - 9
IS  - 1
ER  -

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