Protective and Curative Potentials of <i>Sida corymbosa</i> Extract Against Antioxidant Depletion in Rats Administered Carbon Tetrachloride

Charles Chijioke Dike; Francis Chukwuemeka Ezeonu; Chinenye Enoch Oguazu; Emmanuel Nonso Ezeokafor; Hugh Clifford Chima Maduka; Aloysius Ngozika Okpogba; Akpoghene Eyeghre Onoriode; Sunday Jacob Asebioyo; Emilia Chika Nwankwo; Ebelechukwu Nwaoma Ihekwoaba; Aronu Cecilia Nkechi

doi:doi:10.11648/j.ajbls.20210902.11

Protective and Curative Potentials of Sida corymbosa Extract Against Antioxidant Depletion in Rats Administered Carbon Tetrachloride

Charles Chijioke Dike, Francis Chukwuemeka Ezeonu, Chinenye Enoch Oguazu, Emmanuel Nonso Ezeokafor, Hugh Clifford Chima Maduka, Aloysius Ngozika Okpogba, Akpoghene Eyeghre Onoriode, Sunday Jacob Asebioyo, Emilia Chika Nwankwo, Ebelechukwu Nwaoma Ihekwoaba, Aronu Cecilia Nkechi

Published in American Journal of Biomedical and Life Sciences (Volume 9, Issue 2)

Received: 27 May 2020 Accepted: 15 June 2020 Published: 4 March 2021

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Abstract

Sida corymbosa plant is one of medicinal plants used in many parts of Africa, including Nigeria for the treatment of human diseases. The purpose of this work was to investigate the antioxidant effects of Sida corymbosa ethanolic leaf extract on rats administered carbon tetrachloride (CCl₄). One hundred and sixty male-rats between 170-180 g were used. They were grouped into 8 major groups (groups A-H) and 4 subgroups (A₁-H₄) in each group making it 32 subgroups of 5 rats each. The major groups comprise A (positive control- normal rats giving feed and water only and left for 7-28 d), B (negative control-those administered CCl₄ only at 0.4 ml/kgbw and left for 7-28 d), C-H (treatment groups-those administered extract before and after CCl₄ administration at 5,000, 3,000 and 1,000 mg/kgbw for 7-28 d). Antioxidant assays were determined using UV-VIS spectrophotometer (model 752g, China). Results of serum antioxidant assays done revealed that levels of serum reduced glutathione (GSH), glutathione peroxidase (GPx), catalase (CAT), supraoxide dismutase (SOD) and total antioxidant capacity (TAC) increased significantly (P<0.05) in the treatment groups from 7-28 d, when compared with the negative control. Oxidative assay carried out showed that serum malondialdehyde (MDA) levels were significantly lower (P<0.05) in treatment groups than in negative control groups. These may be suggesting that the extract may have improved the antioxidant levels of the rats. Sida corymbosa ethanolic leaf extract may, therefore, have positive effects on antioxidant status of rats administered CCl₄.

Published in	American Journal of Biomedical and Life Sciences (Volume 9, Issue 2)
DOI	10.11648/j.ajbls.20210902.11
Page(s)	97-110
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Sida Species, Antioxidant Results in Rats, Oxidative Stress

References

[1]	Dike C. C., F. C. Ezeonu, H. C. C. Maduka, E. N. Ezeokafor and C. E. Oguazu (2018a). Hepatoprotective potentials of Sida corymbosa ethanolic leaf extract against carbon tetrachloride-induced hepatotoxicity on male Albino Wistar rats. Journal of Pharmacognosy and Phytochemistry 7, 1266-1275.
[2]	Dike C. C., F. C. Ezeonu, H. C. C. Maduka, E. N. Ezeokafor and C. E. Oguazu (2018b). Hepatotherapeutic potentials of Sida corymbosa (Wire weed) ethanolic leaf extract against carbon tetrachloride (CCl₄)-induced acute hepatotoxicity in male Albino rats. European Journal of Pharmaceutical and Medical Research 5, 146-155.
[3]	Dike C. C., F. C. Ezeonu, H. C. C. Maduka, E. N. Ezeokafor (2018c). Phytochemical and elemental analysis of Sida corymbosa leaf and root extracts. International Journal of Chemical Sciences 24, 1-9.
[4]	Palaksha M. N., K. Ravishankar and S. V. Girija (2016). Comparative in vitro antioxidant activity on Melochia corchorifolia, Sida acuta and Saccharum officinarum leaf extracts and their phenolic contents. Saudi Journal of Medical and Pharmaceutical Sciences 2, 198-204.
[5]	Maduka H. C. C., C. E. Ugwu, C. C. Dike, A. N. Okpogba, P. N. Ogueche, M. S. Ali and SS. Bakare (2017b). Tino J. In vitro free radical scavenging and antioxidant activities of aqueous extracts of Cateva adansonii (Three leaf plant). Journal of Advances in Medical and Pharmaceutical Sciences 15, 1-8.
[6]	Jian-Ming L. O., H. L. Peter, Y. Qizhi and C (2010). Changyi Chemical and molecular Mechanisms of antioxidants: experimental approaches and model systems. Journal of Cell and Molecular Medicine 14, 840-860.
[7]	Obi U. L., A. Norah, C. Okafor, C. E. Dioka and S. C. Meludu (2017). Evaluation of total antioxidant status, supraoxide dismutase and malondialdehyde in apparently healthy active tobacco smokers in Nnewi Metropolis, South-East, Nigeria Journal of Scientific Innovation and Research 6, 105-112.
[8]	Maduka H. C. C., A. N. Okpogba, C. E, Ugwu, C. C. Dike P. N. Ogueche, D. T, Onwuzuruike and D. C. Ibe (2015). Phytochemical, antioxidant and microbial inhibitory effects of Spondias mombin leaf and stem bark extracts. Journal of Pharmaceutical and Biological Sciences 9, 14-17.
[9]	Maher A. A., A. E. Mohamed and A Aya (2015). The role of Ficus carica leaf extract in modulation of the experimentally-induced hepatotoxicity damage in male rats. International Journal of Advanced Research 3, 572-588.
[10]	Kebe E. O., U. Gabriel C. Kelechi and O. I (2015). Anozeng The effect of Sida acuta on glycogen profile of adult Wistar rat. Global Journal of Biology, Agriculture and Health Sciences 4, 52-55.
[11]	Philip N. N., E. Guillaume D. E. and V. Peter (2018). Antony Contrasting nickel and zinc hyperaccumulation in subspecies of Dichapetalum gelonioides from Southeast Asia. Scientific Reports 8, 9659.
[12]	Maduka H. C. C., E. I. Akubugwo, O. Olorunipe, A. N. Okpogba, A. A. Maduka, C. U. Ayuru, C. E. Ugwu, C. C. Dike and P. N. Ogueche (2016). Elemental composition and phytochemical screening of aqueous leaf extract and stem bark extract of Crateva adansonii. International Journal of Biochemistry Research and Review 10, 1-7.
[13]	Adepoju A A., I. Desalu, W. L. Adeyemo and M. O. Akintimoye (2011). Airway and respiratory complications in children undergoing cleft lip and palate repair. Ghana Medical Journal 10, 44: 1-10.
[14]	Mehran R., V. R. Sunil, L. Deepak, C. Michael, C. Adriano and E. John (2010). Standardized bleeding, definitions for cardiovascular clinical trials: a consensus report from the bleeding academic research consortium. Circulation 123, 2736-2747.
[15]	Biswanath D., D. Niranjan, D. Subhajit, D. Manikarna, and S. Indrajit (2012). The genus Sida L.-traditional medicine: Its ethnopharmacological, phytochemical and pharmacological data for commercial exploitation in herbal drugs industry. J Ethnopharmacology 15, 135-176.
[16]	Ajithabai MD., SP. Sunitha, S. Balachandran and G. Jayakumar (2015). The use of Sida plant in the preparation of Nayapayam kashayam. International Journal of Research and Applied Sciences 3, 99-102.
[17]	Sundarganapath R., V. Niraimathi, T. Ananda, M. Jambulingm, B. Narasimhan and D. Aakash (2013). Phytochemical studies and pharmacological screening of Sida rhombifalia Linn. Hygeia. Journal of Dental Medicine 5, 19-22.
[18]	Lucy BJ., and A. Mercy (2015). Evaluation of the haemostatic activities of Sida corymbosa in rats. British Journal of Pharmaceutical Research 5, 431- 436.
[19]	Mahesh T., M. Shashank, R. S. Kalidhindi, S. P Dhanabai and D. N. Ashish (2016). In vitro and in vivio evaluation of Sida acuta burm. f. (Malvaceae) for its antioxidant and anticancer activity. Der Pharmachemical 8, 396-402.
[20]	Abat J., S. Kumar and A. Mohanty (2017). Ethnomedicinal, phytochemical and ethnopharmacological aspects of four medicinal plants of malvaceae used in Indian traditional Medicine: A review. Medicines 75, 1-8.
[21]	Lucy J., Y. and Y. I. Tijani (2014). Christianah Anti-ulcer and wound healing activities of Sida corymbosa in rats. African Journal of Traditional, Complementary and Alternative Medicine 11, 87-92.
[22]	Shwetha K. and G. M. Sudha (2012). In vitro free radical scavenging activity of aqueous extract from the mycelia of Volvariella volvacea (Bulliard Ex Fries) Singer. International Journal of Current Pharmaceutical Research 4, 94-100.
[23]	Kalita P., T. K. Barman, T. K. Pal and R. Kalita (2013). Estimation of total flavonoids content (TFC) and antioxidant activities of methanolic whole plant extract of Biophytum sensitivum Linn. Journal of Drug Delivery and Therapeutics 3, 33-37.
[24]	Aliyu R., Z. S. C. Okoye and W. T. Shier (2012). The hepatoproctective cytochrome P-450 enzyme inhibitor isolated from Nigerian medicinal plant Cochlospermum planchonii is a zinc salt. Journal of Ethnopharmacology 48, 89-97.
[25]	Enegide C., A. David and S. A. (2013). Fidelis A new Method for determining acute toxicity in animal models. Toxicology International. 20: 224-226.
[26]	WHO (2004). Recommendations for the preparation, characterization and establishment of International and other biological reference stardards. WHO Experimental Committee on biological standardization. Fifty-fifth report. WHO Technical Report Series No 932. http://www.whoint/blood products/norms/en/ (accessed 13 July 2004).
[27]	Beutiler E. (1993). Enzyme replacement therapy for Gaucher’s disease. Baillieres Clinical Haematology 10, 751-763.
[28]	Hadwan M. H and H. N. (2016). Abed Data supporting the estimation of catalase activity. Data Brief. 6: 194-199.
[29]	Rotruck J. T, A. L. Pope, H. E. Ganther, A. B. Swanson, D. G. Hafeman and J. H. William (1993). Selenium Biochemical Role as a component of glutathione peroxidase. Science 179, 588-590.
[30]	Benzie I. F. F. and J. J. Strain [1996]. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power. The FRAP assay. Analytical Biochemistry 239, 70- 76.
[31]	Alam Z. and U. C. Fareed (2016). A simple spectrophotometric method for the determination of thiobarbitutric acid reactive substance in fried fast foods. Journal of Analytical Methods in Chemistry 8, 1-5.
[32]	Maduka H. C. C., A. A. Maduka, C. U. Aguoru, A. Myke-Mbata, J. O. Ikwue, A. N. Okpogba, Ugwu C. E, C. C. Dike, P. N. Ogueche, D. O. Ochalefu, U. Nath, J. Yakubu and E. Shatu (2017a). Phytochemical screening, spectrophotometric determination of total carotenoids, chlorophyll a and b components of Crateva adansonii (Three-leaf Plant). Journal of Advanced Medical and Pharmaceutical Sciences 15, 1-6.
[33]	Perumalsamy M., D. Subburaj, C. P. Palanisamy, K. G. Velliyur and Kannappan (2016). Phytochemical Screening and free radical scavenging activity of chloroform extract of Sida acuta Burn F. International Journal of Pharmacognosy and phytochemical Research 8, 663-667.

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APA Style

Charles Chijioke Dike, Francis Chukwuemeka Ezeonu, Chinenye Enoch Oguazu, Emmanuel Nonso Ezeokafor, Hugh Clifford Chima Maduka, et al. (2021). Protective and Curative Potentials of Sida corymbosa Extract Against Antioxidant Depletion in Rats Administered Carbon Tetrachloride. American Journal of Biomedical and Life Sciences, 9(2), 97-110. https://doi.org/10.11648/j.ajbls.20210902.11

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ACS Style

Charles Chijioke Dike; Francis Chukwuemeka Ezeonu; Chinenye Enoch Oguazu; Emmanuel Nonso Ezeokafor; Hugh Clifford Chima Maduka, et al. Protective and Curative Potentials of Sida corymbosa Extract Against Antioxidant Depletion in Rats Administered Carbon Tetrachloride. Am. J. Biomed. Life Sci. 2021, 9(2), 97-110. doi: 10.11648/j.ajbls.20210902.11

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AMA Style

Charles Chijioke Dike, Francis Chukwuemeka Ezeonu, Chinenye Enoch Oguazu, Emmanuel Nonso Ezeokafor, Hugh Clifford Chima Maduka, et al. Protective and Curative Potentials of Sida corymbosa Extract Against Antioxidant Depletion in Rats Administered Carbon Tetrachloride. Am J Biomed Life Sci. 2021;9(2):97-110. doi: 10.11648/j.ajbls.20210902.11

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@article{10.11648/j.ajbls.20210902.11,
  author = {Charles Chijioke Dike and Francis Chukwuemeka Ezeonu and Chinenye Enoch Oguazu and Emmanuel Nonso Ezeokafor and Hugh Clifford Chima Maduka and Aloysius Ngozika Okpogba and Akpoghene Eyeghre Onoriode and Sunday Jacob Asebioyo and Emilia Chika Nwankwo and Ebelechukwu Nwaoma Ihekwoaba and Aronu Cecilia Nkechi},
  title = {Protective and Curative Potentials of Sida corymbosa Extract Against Antioxidant Depletion in Rats Administered Carbon Tetrachloride},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {9},
  number = {2},
  pages = {97-110},
  doi = {10.11648/j.ajbls.20210902.11},
  url = {https://doi.org/10.11648/j.ajbls.20210902.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20210902.11},
  abstract = {Sida corymbosa plant is one of medicinal plants used in many parts of Africa, including Nigeria for the treatment of human diseases. The purpose of this work was to investigate the antioxidant effects of Sida corymbosa ethanolic leaf extract on rats administered carbon tetrachloride (CCl4). One hundred and sixty male-rats between 170-180 g were used. They were grouped into 8 major groups (groups A-H) and 4 subgroups (A1-H4) in each group making it 32 subgroups of 5 rats each. The major groups comprise A (positive control- normal rats giving feed and water only and left for 7-28 d), B (negative control-those administered CCl4 only at 0.4 ml/kgbw and left for 7-28 d), C-H (treatment groups-those administered extract before and after CCl4 administration at 5,000, 3,000 and 1,000 mg/kgbw for 7-28 d). Antioxidant assays were determined using UV-VIS spectrophotometer (model 752g, China). Results of serum antioxidant assays done revealed that levels of serum reduced glutathione (GSH), glutathione peroxidase (GPx), catalase (CAT), supraoxide dismutase (SOD) and total antioxidant capacity (TAC) increased significantly (PSida corymbosa ethanolic leaf extract may, therefore, have positive effects on antioxidant status of rats administered CCl4.},
 year = {2021}
}

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TY  - JOUR
T1  - Protective and Curative Potentials of Sida corymbosa Extract Against Antioxidant Depletion in Rats Administered Carbon Tetrachloride
AU  - Charles Chijioke Dike
AU  - Francis Chukwuemeka Ezeonu
AU  - Chinenye Enoch Oguazu
AU  - Emmanuel Nonso Ezeokafor
AU  - Hugh Clifford Chima Maduka
AU  - Aloysius Ngozika Okpogba
AU  - Akpoghene Eyeghre Onoriode
AU  - Sunday Jacob Asebioyo
AU  - Emilia Chika Nwankwo
AU  - Ebelechukwu Nwaoma Ihekwoaba
AU  - Aronu Cecilia Nkechi
Y1  - 2021/03/04
PY  - 2021
N1  - https://doi.org/10.11648/j.ajbls.20210902.11
DO  - 10.11648/j.ajbls.20210902.11
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 97
EP  - 110
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20210902.11
AB  - Sida corymbosa plant is one of medicinal plants used in many parts of Africa, including Nigeria for the treatment of human diseases. The purpose of this work was to investigate the antioxidant effects of Sida corymbosa ethanolic leaf extract on rats administered carbon tetrachloride (CCl4). One hundred and sixty male-rats between 170-180 g were used. They were grouped into 8 major groups (groups A-H) and 4 subgroups (A1-H4) in each group making it 32 subgroups of 5 rats each. The major groups comprise A (positive control- normal rats giving feed and water only and left for 7-28 d), B (negative control-those administered CCl4 only at 0.4 ml/kgbw and left for 7-28 d), C-H (treatment groups-those administered extract before and after CCl4 administration at 5,000, 3,000 and 1,000 mg/kgbw for 7-28 d). Antioxidant assays were determined using UV-VIS spectrophotometer (model 752g, China). Results of serum antioxidant assays done revealed that levels of serum reduced glutathione (GSH), glutathione peroxidase (GPx), catalase (CAT), supraoxide dismutase (SOD) and total antioxidant capacity (TAC) increased significantly (PSida corymbosa ethanolic leaf extract may, therefore, have positive effects on antioxidant status of rats administered CCl4.
VL  - 9
IS  - 2
ER  -

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Author Information

Charles Chijioke Dike

Department of Human Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria
Francis Chukwuemeka Ezeonu

Department of Applied Biochemistry, Faculty of Biosciences, Nnamdi Azikiwe University, Awka, Nigeria
Chinenye Enoch Oguazu

Department of Applied Biochemistry, Faculty of Biosciences, Nnamdi Azikiwe University, Awka, Nigeria
Emmanuel Nonso Ezeokafor

Department of Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria
Hugh Clifford Chima Maduka

Department of Human Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria
Aloysius Ngozika Okpogba

Department of Human Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria
Akpoghene Eyeghre Onoriode

Department of Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria
Sunday Jacob Asebioyo

Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria
Emilia Chika Nwankwo

Department of Human Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria
Ebelechukwu Nwaoma Ihekwoaba

Department of Environmental Health Sciences, Faculty of Health Sciences and Technology, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria
Aronu Cecilia Nkechi

Department of Environmental Health Sciences, Faculty of Health Sciences and Technology, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria

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APA Style

Charles Chijioke Dike, Francis Chukwuemeka Ezeonu, Chinenye Enoch Oguazu, Emmanuel Nonso Ezeokafor, Hugh Clifford Chima Maduka, et al. (2021). Protective and Curative Potentials of Sida corymbosa Extract Against Antioxidant Depletion in Rats Administered Carbon Tetrachloride. American Journal of Biomedical and Life Sciences, 9(2), 97-110. https://doi.org/10.11648/j.ajbls.20210902.11

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Charles Chijioke Dike; Francis Chukwuemeka Ezeonu; Chinenye Enoch Oguazu; Emmanuel Nonso Ezeokafor; Hugh Clifford Chima Maduka, et al. Protective and Curative Potentials of Sida corymbosa Extract Against Antioxidant Depletion in Rats Administered Carbon Tetrachloride. Am. J. Biomed. Life Sci. 2021, 9(2), 97-110. doi: 10.11648/j.ajbls.20210902.11

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AMA Style

Charles Chijioke Dike, Francis Chukwuemeka Ezeonu, Chinenye Enoch Oguazu, Emmanuel Nonso Ezeokafor, Hugh Clifford Chima Maduka, et al. Protective and Curative Potentials of Sida corymbosa Extract Against Antioxidant Depletion in Rats Administered Carbon Tetrachloride. Am J Biomed Life Sci. 2021;9(2):97-110. doi: 10.11648/j.ajbls.20210902.11

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@article{10.11648/j.ajbls.20210902.11,
  author = {Charles Chijioke Dike and Francis Chukwuemeka Ezeonu and Chinenye Enoch Oguazu and Emmanuel Nonso Ezeokafor and Hugh Clifford Chima Maduka and Aloysius Ngozika Okpogba and Akpoghene Eyeghre Onoriode and Sunday Jacob Asebioyo and Emilia Chika Nwankwo and Ebelechukwu Nwaoma Ihekwoaba and Aronu Cecilia Nkechi},
  title = {Protective and Curative Potentials of Sida corymbosa Extract Against Antioxidant Depletion in Rats Administered Carbon Tetrachloride},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {9},
  number = {2},
  pages = {97-110},
  doi = {10.11648/j.ajbls.20210902.11},
  url = {https://doi.org/10.11648/j.ajbls.20210902.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20210902.11},
  abstract = {Sida corymbosa plant is one of medicinal plants used in many parts of Africa, including Nigeria for the treatment of human diseases. The purpose of this work was to investigate the antioxidant effects of Sida corymbosa ethanolic leaf extract on rats administered carbon tetrachloride (CCl4). One hundred and sixty male-rats between 170-180 g were used. They were grouped into 8 major groups (groups A-H) and 4 subgroups (A1-H4) in each group making it 32 subgroups of 5 rats each. The major groups comprise A (positive control- normal rats giving feed and water only and left for 7-28 d), B (negative control-those administered CCl4 only at 0.4 ml/kgbw and left for 7-28 d), C-H (treatment groups-those administered extract before and after CCl4 administration at 5,000, 3,000 and 1,000 mg/kgbw for 7-28 d). Antioxidant assays were determined using UV-VIS spectrophotometer (model 752g, China). Results of serum antioxidant assays done revealed that levels of serum reduced glutathione (GSH), glutathione peroxidase (GPx), catalase (CAT), supraoxide dismutase (SOD) and total antioxidant capacity (TAC) increased significantly (PSida corymbosa ethanolic leaf extract may, therefore, have positive effects on antioxidant status of rats administered CCl4.},
 year = {2021}
}

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TY  - JOUR
T1  - Protective and Curative Potentials of Sida corymbosa Extract Against Antioxidant Depletion in Rats Administered Carbon Tetrachloride
AU  - Charles Chijioke Dike
AU  - Francis Chukwuemeka Ezeonu
AU  - Chinenye Enoch Oguazu
AU  - Emmanuel Nonso Ezeokafor
AU  - Hugh Clifford Chima Maduka
AU  - Aloysius Ngozika Okpogba
AU  - Akpoghene Eyeghre Onoriode
AU  - Sunday Jacob Asebioyo
AU  - Emilia Chika Nwankwo
AU  - Ebelechukwu Nwaoma Ihekwoaba
AU  - Aronu Cecilia Nkechi
Y1  - 2021/03/04
PY  - 2021
N1  - https://doi.org/10.11648/j.ajbls.20210902.11
DO  - 10.11648/j.ajbls.20210902.11
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 97
EP  - 110
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20210902.11
AB  - Sida corymbosa plant is one of medicinal plants used in many parts of Africa, including Nigeria for the treatment of human diseases. The purpose of this work was to investigate the antioxidant effects of Sida corymbosa ethanolic leaf extract on rats administered carbon tetrachloride (CCl4). One hundred and sixty male-rats between 170-180 g were used. They were grouped into 8 major groups (groups A-H) and 4 subgroups (A1-H4) in each group making it 32 subgroups of 5 rats each. The major groups comprise A (positive control- normal rats giving feed and water only and left for 7-28 d), B (negative control-those administered CCl4 only at 0.4 ml/kgbw and left for 7-28 d), C-H (treatment groups-those administered extract before and after CCl4 administration at 5,000, 3,000 and 1,000 mg/kgbw for 7-28 d). Antioxidant assays were determined using UV-VIS spectrophotometer (model 752g, China). Results of serum antioxidant assays done revealed that levels of serum reduced glutathione (GSH), glutathione peroxidase (GPx), catalase (CAT), supraoxide dismutase (SOD) and total antioxidant capacity (TAC) increased significantly (PSida corymbosa ethanolic leaf extract may, therefore, have positive effects on antioxidant status of rats administered CCl4.
VL  - 9
IS  - 2
ER  -

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