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Association of Polymorphisms at PRLR Gene Loci with Litter Size in Haimen Goat

Received: 29 July 2016     Accepted: 8 August 2016     Published: 29 August 2016
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Abstract

Haimen goat is an exclusive and relatively rare indigenous Chinese goat breed that can produce high quality Brush Hair specialized in making valuable writing brush. To enlarge quickly the population size of Haimen goat, certain genetic markers that can improve litter size are needed in the breeding process. To search for suitable genetic markers related to litter size, the polymorphisms at the PRLR gene loci were investigated by PCR and single-strand conformation polymorphisms (SSCP) in Haimen goat, and their associations with litter size were analyzed by genetic tools. Results showed that two alleles are found on PRLR gene Intron 1, with gene frequencies was 0.59 and 0.41, respectively; the heterozygosity (He), effective allele number (Ne) and polymorphic information content (PIC) in Haimen Goat based on Intron 1 was 0.4838, 1.9372 and 0.3668, respectively; Two alleles were found on PRLR gene Intron 2, and gene frequencies was 0.79 and 0.21, respectively. He, Ne and PIC in Haimen Goat on Intron 2 was 0.3318, 1.4966 and 0.2768, respectively. The genetic variation indicated that the Haimen goat population was moderately polymorphic. The least square analysis and multicomparison of PRLR gene loci and litter size demonstrated that the SNP (A35G) on Intron 2 of the PRLR gene affected extremely significantly on litter size, with CC as the advantageous genotype for the trait of litter size; while the SNP (T95C) on Intron 1 had no significantly effect on litter size in Haimen goat. The results would provide some evidence for SNP (A35G) on Intron 2 of the PRLR gene as a candidate marker for the trait of litter size in goat.

Published in Biochemistry and Molecular Biology (Volume 1, Issue 2)
DOI 10.11648/j.bmb.20160102.13
Page(s) 23-26
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Goat, PRLR, Litter Size, Polymorphism, PCR-SSCP

References
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[2] Drogemuller C, Hamann H, Distl O. (2001) Candidate gene markers for litter size in different German Pig lines. J. Anim. Sci., 79: 2565-2570.
[3] Van Rens B T, Evans G J, Van der Lende T. (2003) Components of litter size in gilts with different prolactin receptor genotypes. Theriogenology, 59 (3-4):915-926.
[4] XU Ning-Ying, ZHANG Sheng-Qiao, PENG Shu-Hong. (2003) Investigation on the Distribution and Their Effects on Reproduction Traits of Three Major Genes in Jinhua Pigs. Acta Genetica Sinica, 30 (12): 1090-1096.
[5] CHU Ming-xing, ZHANG Gen-xi, WANG Jin-yu, FANG Li, YE Su-cheng. (2008) Polymorphism of Prolactin Receptor Gene and Its Relationship with Litter Size of Some Goat Breeds. Journal of Agricultural Biotechnology. 16 (4):725-726.
[6] ZHANG Gen-Xi, CHU Ming-Xing, WANG Jin-Yu, FANG Li, YE Su-Cheng. (2007) Polymorphism of exon10 of prolactin receptor gene and its relationship with prolificacy of Jining Grey goats. HEREDITAS, 29 (3): 329-336.
[7] Mullenbach R, Lagoda PJ, Welter C (1989) An efficient salt chloroform extraction of DNA from blood and tissue. Trends Genetics, 5:391.
[8] MU Yu-lian, CHU Ming-xing, SUN Shaohua, FANG Li, YE Sucheng. (2006) PCR-SSCP Analysis on Prolactin Receptor Gene in Sheep. Acta Veterinaria et Zootechnica Sinica, 37 (10): 956-960.
[9] ChunLei Zhang, Yanhong Wang, Hong Chen, XianYong Lan, ChuZhao Lei. (2007) Enhance the efficiency of single-strand conformation polymorphism analysis by short polyacrylamide gel and modified silver staining. Anal Biochem, 365: 286–287.
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Cite This Article
  • APA Style

    Dejun Ji, Guohu Cheng, Yongjun Li, Hao Zhang, Haiyan Guo, et al. (2016). Association of Polymorphisms at PRLR Gene Loci with Litter Size in Haimen Goat. Biochemistry and Molecular Biology, 1(2), 23-26. https://doi.org/10.11648/j.bmb.20160102.13

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    ACS Style

    Dejun Ji; Guohu Cheng; Yongjun Li; Hao Zhang; Haiyan Guo, et al. Association of Polymorphisms at PRLR Gene Loci with Litter Size in Haimen Goat. Biochem. Mol. Biol. 2016, 1(2), 23-26. doi: 10.11648/j.bmb.20160102.13

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    AMA Style

    Dejun Ji, Guohu Cheng, Yongjun Li, Hao Zhang, Haiyan Guo, et al. Association of Polymorphisms at PRLR Gene Loci with Litter Size in Haimen Goat. Biochem Mol Biol. 2016;1(2):23-26. doi: 10.11648/j.bmb.20160102.13

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  • @article{10.11648/j.bmb.20160102.13,
      author = {Dejun Ji and Guohu Cheng and Yongjun Li and Hao Zhang and Haiyan Guo and Miaoying Cai},
      title = {Association of Polymorphisms at PRLR Gene Loci with Litter Size in Haimen Goat},
      journal = {Biochemistry and Molecular Biology},
      volume = {1},
      number = {2},
      pages = {23-26},
      doi = {10.11648/j.bmb.20160102.13},
      url = {https://doi.org/10.11648/j.bmb.20160102.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20160102.13},
      abstract = {Haimen goat is an exclusive and relatively rare indigenous Chinese goat breed that can produce high quality Brush Hair specialized in making valuable writing brush. To enlarge quickly the population size of Haimen goat, certain genetic markers that can improve litter size are needed in the breeding process. To search for suitable genetic markers related to litter size, the polymorphisms at the PRLR gene loci were investigated by PCR and single-strand conformation polymorphisms (SSCP) in Haimen goat, and their associations with litter size were analyzed by genetic tools. Results showed that two alleles are found on PRLR gene Intron 1, with gene frequencies was 0.59 and 0.41, respectively; the heterozygosity (He), effective allele number (Ne) and polymorphic information content (PIC) in Haimen Goat based on Intron 1 was 0.4838, 1.9372 and 0.3668, respectively; Two alleles were found on PRLR gene Intron 2, and gene frequencies was 0.79 and 0.21, respectively. He, Ne and PIC in Haimen Goat on Intron 2 was 0.3318, 1.4966 and 0.2768, respectively. The genetic variation indicated that the Haimen goat population was moderately polymorphic. The least square analysis and multicomparison of PRLR gene loci and litter size demonstrated that the SNP (A35G) on Intron 2 of the PRLR gene affected extremely significantly on litter size, with CC as the advantageous genotype for the trait of litter size; while the SNP (T95C) on Intron 1 had no significantly effect on litter size in Haimen goat. The results would provide some evidence for SNP (A35G) on Intron 2 of the PRLR gene as a candidate marker for the trait of litter size in goat.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - Association of Polymorphisms at PRLR Gene Loci with Litter Size in Haimen Goat
    AU  - Dejun Ji
    AU  - Guohu Cheng
    AU  - Yongjun Li
    AU  - Hao Zhang
    AU  - Haiyan Guo
    AU  - Miaoying Cai
    Y1  - 2016/08/29
    PY  - 2016
    N1  - https://doi.org/10.11648/j.bmb.20160102.13
    DO  - 10.11648/j.bmb.20160102.13
    T2  - Biochemistry and Molecular Biology
    JF  - Biochemistry and Molecular Biology
    JO  - Biochemistry and Molecular Biology
    SP  - 23
    EP  - 26
    PB  - Science Publishing Group
    SN  - 2575-5048
    UR  - https://doi.org/10.11648/j.bmb.20160102.13
    AB  - Haimen goat is an exclusive and relatively rare indigenous Chinese goat breed that can produce high quality Brush Hair specialized in making valuable writing brush. To enlarge quickly the population size of Haimen goat, certain genetic markers that can improve litter size are needed in the breeding process. To search for suitable genetic markers related to litter size, the polymorphisms at the PRLR gene loci were investigated by PCR and single-strand conformation polymorphisms (SSCP) in Haimen goat, and their associations with litter size were analyzed by genetic tools. Results showed that two alleles are found on PRLR gene Intron 1, with gene frequencies was 0.59 and 0.41, respectively; the heterozygosity (He), effective allele number (Ne) and polymorphic information content (PIC) in Haimen Goat based on Intron 1 was 0.4838, 1.9372 and 0.3668, respectively; Two alleles were found on PRLR gene Intron 2, and gene frequencies was 0.79 and 0.21, respectively. He, Ne and PIC in Haimen Goat on Intron 2 was 0.3318, 1.4966 and 0.2768, respectively. The genetic variation indicated that the Haimen goat population was moderately polymorphic. The least square analysis and multicomparison of PRLR gene loci and litter size demonstrated that the SNP (A35G) on Intron 2 of the PRLR gene affected extremely significantly on litter size, with CC as the advantageous genotype for the trait of litter size; while the SNP (T95C) on Intron 1 had no significantly effect on litter size in Haimen goat. The results would provide some evidence for SNP (A35G) on Intron 2 of the PRLR gene as a candidate marker for the trait of litter size in goat.
    VL  - 1
    IS  - 2
    ER  - 

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Author Information
  • Key Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, China

  • Key Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, China

  • Key Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, China

  • Key Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, China

  • Key Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, China

  • Key Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, China

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