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Comparative Analysis of Genetic Diversity Among Bacillus Thuringiensis and Bacillus Sphaericus and Their Fusants Using Molecular Markers

Received: 10 November 2018     Accepted: 7 December 2018     Published: 10 January 2019
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Abstract

Ten genetically stable fusants strains were obtained as a result of protoplast fusion technique between Bacillus sphaericus (Bs) and Bacillus thuringiensis (Bt), six of them produced from, Bs GHAI Bt 66 Ia experiment (fusants no. 1, 2, 16, 26, 31 and 34) and four fusants produced from Bs GHAI Bt I977experiment (fusants no.1, 2, 17 and 25). Two molecular marker systems, four ISSR primers (Inter-Simple Sequence Repeat) and seven SCoT primers (Start Codon Targeted Polymorphism) were employed for detection of genetic variation between the parents and their fusants. Four ISSR primers and seven SCoT detected 55and 97 amplicons, among which 92.696% and 81.948% bands were polymorphic respectively. The genetic similarity values among the species were calculated based on ISSR and SCoT profiles which ranged from 0.50 to 0.83 and 0.59 to 0.90 respectively. Based on the marker analysis, the three parents and the ten fusants were clustered into three major groups for ISSR primer and SCoT primers. Cluster analysis were performed to construct a dendrogram of the Bacillus Strains and their fusants and also reveals high genetic variation among the parents and their fusants. It could be concluded that each type of the two molecular marker approaches of DNA analysis could identify the different between the parents, and all of their fusants under investigation have probably originated from closely related ancestors and possess high degree of genetic similarity.

Published in Biochemistry and Molecular Biology (Volume 3, Issue 5)
DOI 10.11648/j.bmb.20180305.11
Page(s) 63-70
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Bacillus Thuringiensis, Bacillus Sphaericus, Genetic Diversity, ISSR and SCoT Marker

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    El-Kawokgy Tahany Mohamed Abou El-Fath, Ibrahim Shafik Darwish, Ashraf Gamil Attallah. (2019). Comparative Analysis of Genetic Diversity Among Bacillus Thuringiensis and Bacillus Sphaericus and Their Fusants Using Molecular Markers. Biochemistry and Molecular Biology, 3(5), 63-70. https://doi.org/10.11648/j.bmb.20180305.11

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    El-Kawokgy Tahany Mohamed Abou El-Fath; Ibrahim Shafik Darwish; Ashraf Gamil Attallah. Comparative Analysis of Genetic Diversity Among Bacillus Thuringiensis and Bacillus Sphaericus and Their Fusants Using Molecular Markers. Biochem. Mol. Biol. 2019, 3(5), 63-70. doi: 10.11648/j.bmb.20180305.11

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    AMA Style

    El-Kawokgy Tahany Mohamed Abou El-Fath, Ibrahim Shafik Darwish, Ashraf Gamil Attallah. Comparative Analysis of Genetic Diversity Among Bacillus Thuringiensis and Bacillus Sphaericus and Their Fusants Using Molecular Markers. Biochem Mol Biol. 2019;3(5):63-70. doi: 10.11648/j.bmb.20180305.11

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  • @article{10.11648/j.bmb.20180305.11,
      author = {El-Kawokgy Tahany Mohamed Abou El-Fath and Ibrahim Shafik Darwish and Ashraf Gamil Attallah},
      title = {Comparative Analysis of Genetic Diversity Among Bacillus Thuringiensis and Bacillus Sphaericus and Their Fusants Using Molecular Markers},
      journal = {Biochemistry and Molecular Biology},
      volume = {3},
      number = {5},
      pages = {63-70},
      doi = {10.11648/j.bmb.20180305.11},
      url = {https://doi.org/10.11648/j.bmb.20180305.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20180305.11},
      abstract = {Ten genetically stable fusants strains were obtained as a result of protoplast fusion technique between Bacillus sphaericus (Bs) and Bacillus thuringiensis (Bt), six of them produced from, Bs GHAI Bt 66 Ia experiment (fusants no. 1, 2, 16, 26, 31 and 34) and four fusants produced from Bs GHAI Bt I977experiment (fusants no.1, 2, 17 and 25). Two molecular marker systems, four ISSR primers (Inter-Simple Sequence Repeat) and seven SCoT primers (Start Codon Targeted Polymorphism) were employed for detection of genetic variation between the parents and their fusants. Four ISSR primers and seven SCoT detected 55and 97 amplicons, among which 92.696% and 81.948% bands were polymorphic respectively. The genetic similarity values among the species were calculated based on ISSR and SCoT profiles which ranged from 0.50 to 0.83 and 0.59 to 0.90 respectively. Based on the marker analysis, the three parents and the ten fusants were clustered into three major groups for ISSR primer and SCoT primers. Cluster analysis were performed to construct a dendrogram of the Bacillus Strains and their fusants and also reveals high genetic variation among the parents and their fusants. It could be concluded that each type of the two molecular marker approaches of DNA analysis could identify the different between the parents, and all of their fusants under investigation have probably originated from closely related ancestors and possess high degree of genetic similarity.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - Comparative Analysis of Genetic Diversity Among Bacillus Thuringiensis and Bacillus Sphaericus and Their Fusants Using Molecular Markers
    AU  - El-Kawokgy Tahany Mohamed Abou El-Fath
    AU  - Ibrahim Shafik Darwish
    AU  - Ashraf Gamil Attallah
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    JF  - Biochemistry and Molecular Biology
    JO  - Biochemistry and Molecular Biology
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    PB  - Science Publishing Group
    SN  - 2575-5048
    UR  - https://doi.org/10.11648/j.bmb.20180305.11
    AB  - Ten genetically stable fusants strains were obtained as a result of protoplast fusion technique between Bacillus sphaericus (Bs) and Bacillus thuringiensis (Bt), six of them produced from, Bs GHAI Bt 66 Ia experiment (fusants no. 1, 2, 16, 26, 31 and 34) and four fusants produced from Bs GHAI Bt I977experiment (fusants no.1, 2, 17 and 25). Two molecular marker systems, four ISSR primers (Inter-Simple Sequence Repeat) and seven SCoT primers (Start Codon Targeted Polymorphism) were employed for detection of genetic variation between the parents and their fusants. Four ISSR primers and seven SCoT detected 55and 97 amplicons, among which 92.696% and 81.948% bands were polymorphic respectively. The genetic similarity values among the species were calculated based on ISSR and SCoT profiles which ranged from 0.50 to 0.83 and 0.59 to 0.90 respectively. Based on the marker analysis, the three parents and the ten fusants were clustered into three major groups for ISSR primer and SCoT primers. Cluster analysis were performed to construct a dendrogram of the Bacillus Strains and their fusants and also reveals high genetic variation among the parents and their fusants. It could be concluded that each type of the two molecular marker approaches of DNA analysis could identify the different between the parents, and all of their fusants under investigation have probably originated from closely related ancestors and possess high degree of genetic similarity.
    VL  - 3
    IS  - 5
    ER  - 

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Author Information
  • Microbial Genetics Department, National Research Centre, Giza, Egypt

  • Molecular Genetics and Genome Mapping Department, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza, Egypt

  • Microbial Genetics Department, National Research Centre, Giza, Egypt

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