The aim of the present study was to evaluate the effects of the aqueous extract of Tetracarpidium conophorum kernels on semen quality and oxidative stress parameters in male rats. 20 rats were randomly divided into 4 lots of 5 animals each, and lots 1 and 2 were given distilled water and the reference molecule for 28 days, while lots 3 and 4 were treated with the aqueous extract of Tetracarpidium conophorum kernels (250 and 500 mg/kg/po). The results show that administration of aqueous extract of Tetracarpidium conophorum kernels (250 and 500 mg/kg/po) to rats produced significant dose-dependent increases (p < 0.05; p < 0.01) in sperm count and motility compared with the control lot treated with distilled water. On the other hand, pH and sperm vitality showed no significant variation (p > 0.05). Administration of the aqueous extract of Tetracarpidium conophorum kernels at the doses studied resulted in no significant variation (p > 0.05) in MDA and CAT concentrations compared with distilled water. However, there was a significant increase (p < 0.05; p < 0.001) in GSH, and GPX concentrations at the respective doses of 500, 250 and 500 mg/kg of Tetracarpidium conophorum aqueous extract compared to rats given distilled water. Chemical analysis of the aqueous extract of Tetracarpidium conophorum kernels revealed the presence of polyphenols and total flavonoids with anti-free radical and antioxidant effects. These results suggest that the aqueous extract of Tetracarpidium conophorum may have spermatogenic and antioxidant potential.
| Published in | American Journal of BioScience (Volume 11, Issue 6) |
| DOI | 10.11648/j.ajbio.20231106.15 |
| Page(s) | 164-170 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2023. Published by Science Publishing Group |
Tetracarpidium conophorum, Oxidative Stress, Semen
| [1] | World Health Organization., 2010. WHO Laboratory Manual for the Examination and Processing of Human Semen (5th edition). Geneva: World Health Organization. |
| [2] | Trussell J., 2013. Optimal diagnosis and medical treatment of male infertility. Semin Reprod Trussell J. Med. 31: 6-235. |
| [3] | Akassa H., Makemba Nkounkou G. S., Nkoua Badzi C., Etou Ossibi A. W., Tamboura H. H., Abena A. A., 2019. Effects of aqueous extract of Pausinystalia yohimbe trunk bark on semen quality and reproductive performance in male wistar rats. Int. J. of Multidisciplinary and Current research. 7: 732-740. |
| [4] | Agarwal A., Said T., 2005. Oxidative stress, DNA damage and apoptosis in male infertility: a clinical approach. BJU Int; 95: 7-503. |
| [5] | Methorst C., Huygue E., 2014. Members of the Andrology and Sexual Medicine Committee of the Association Française d'Urologie. Oxidative stress and male infertility: pathophysiology and therapeutic interest of antioxidants. Progress in Urology, Vol. 24: 4-10. |
| [6] | Ngoula F., Guemdjo Tekam M., Kenfack A., Tadondjou Tchingo CD., Nouboudem S., Ngoumtsop H., Tsafack B., Teguia A., Kamtchouing P., Galeotti M., Tchoumboue J., 2017. Effects of heat stress on some reproductive parameters of male cavie (Cavia porcellus) and mitigation strategies using guava (Psidium guajava) leaves essential oil. J Therm Biol. 64: 67-72. |
| [7] | Kenfack A., Akono N. E., Ngoula F., Nounamo J. G. A., Kamtchouing P., Tchoumboue J., 2018. Post Exposure Effects of Propoxur (Agricultural Pesticide) on Male Fertility in Wistar Rat. Iranian Journal of Toxicology; 12 (3): 21-27. |
| [8] | Landry M. M., Mbongo, J. A., Bimangou J. D. D., Mokondjimobé, E., Iloki L. H., and Abena, A. A., 2019. Latrogenic male infertility with psychotropic drugs in rats: case of haloperidol and clomipramine. International journal of scientific research, (8): 1-3. |
| [9] | Huyghe H., Bonal M., Daudin M., Droupy S., 2013. Sexual dysfunctions and infertility. Progress in Urology Elsevier Masson.; 23: 745- 751. |
| [10] | Akassa H., Peneme B. M. L., Morabandza C. J., Etou ossibi A. W, and Abena A. A, 2022. Evaluation of the androgenic potentialities of the hydro-ethanolic extract of the trunk barks of Strychnos camptoneura (longaniaceae) in the wistar rat. world journal of pharmacy and pharmaceutical sciences; 11: 145-156. |
| [11] | Azonbakin S., Dangbemey P., Osseni R., Yaude, S. A., Kora F., Adovoekpe D., Djego F., Laleye A., Gbaguidi F., 2021. Enquête ethnobotanique sur les plantes utilisées dans le traitement de l’infertilité masculine au Benin. International Journal of Biological and Chemical Sciences. 15. 1667-1677. |
| [12] | Zohoun T. H., Flenon J., 1997. Can traditional medicine and the African pharmacopoeia provide an alternative to the current prohibitive costs of modern medicine? In Pharmacopoeia and Traditional African Medicine. U. B. Press.: Lomé; 3-15. |
| [13] | Byg A., Baslev H., 2001. Diversity and use of palms in Zahamena, eastern Madagascar. Biodivers Conserv., 10: 951–70. |
| [14] | Ntsoumou VM., Adzona PP., Bati J. B., Kengue T., Mabanza -Mbanza B. B., Saboukoulou AJ., Ndinga AF., Guembo J. R., and Banga -Mboko H., 2021. Evaluation of the substitution of Glycine meal max (L.) Merr. (Soja) by the Tetracarpidium conophorum (Mull. Arg.) & Dalz on the performance of laying hens. Int. J. Biol. Chem. Sci.: 15 (6): 2526-2542. |
| [15] | Ossoko J PL., Dzondo G. M., Mvoula T. D., et al., 2021 Assessment of the nutrtional potential of the kernels of the seeds of Tetracarpidium conophorum collected in Lékana in the Départementof Plateaux in the Republic of Congo; J. Bio. Innov. ; 10 (4): 1102- 1110. |
| [16] | Akomolafe SF., Oboh G., Oyeleye S. I., and Tosin A., Olasehinde T. A., 2017. Toxicological Effects of Aqueous Extract From (African Walnut (Tetracarpidium conophorum) Leaves in Rats Journal of Evidence-Based Complementary & Alternative Medicine.; 22 (4): 919-925. |
| [17] | Akassa H., Peneme B. M. L., Etou Ossibi A. W., Morabandza C. J., Kanga M. R., Goma Dinouvanza A., and Abena A. A.,2023. Androgenic and Antioxidant Effects of Ethanolic Extract of Strychnos camptoneura (longaniaceae) Trunk Bark in Male Wistar Rats. American Journal of Pharmacological Sciences, 11(1): 8-14. |
| [18] | Dérivaux V., Ectors F., 1986. Animal domestic reproduction. 3e édition. Cobay Louvain. La Neuve; Liège: 1-439. |
| [19] | Ngoula F. Effects of organophosphorus and carbamate pesticides on some reproduction parameters in the adult male rat. PhD thesis, University of Yaoundé I; Cameroun, 163p. 2008. |
| [20] | Sarika C., 2022. Evaluation of ameliorative effect of quercetin and candesartan in isoprenaline induced myocardial infarctionWorld Journal of Pharmaceutical Research; 11 (2): 1409-1412. |
| [21] | Uhegbu F. O., Imo C., and Ugbogu A. E., 2015. Effect of aqueous extract of Piper guineense seeds on some liver enzymes, antioxidant enzymes and some hematological parameters in albino rats. International Journal of Plant Science and Ecology; 1: 167-171. |
| [22] | Brand Williams W., Cuvelier M. E., and Berset C., 1995. Use of free radical method to evaluate antioxidant activity. Technology 28: 25-30. |
| [23] | Waterhouse A. L., 2001. Determination of total phenolics. Current Protocols in Food Analytical Chemistry; I1.1.1-I1.1.8. |
| [24] | Ngandjui A., Ngaha N. M., Kenmogne H., Koloko B. L., Massoma L. D., 2017. Evaluation of the fertility activity of the aqueous leaves extract of Zanthoxylum macrophylla (Rutaceae) on male rats. J Phytopharmacol; 6 (5): 277-281. |
| [25] | Syed A H., Ahsan H., Furqan N., Yang W., Hafiz A., Rasul S., and Yuanda S., 2018. Evaluation of the Spermatogenic Activity of Polyherbal Formulation in Oligospermic Males. BioMed Research International: 1-10. |
| [26] | Akassa H, Samlan O. A. N., Peneme B. M L., Goma D. A. L., Kanga M. R., and Abena A. A., 2023. Spermatogenic and antiradical activities of the hydro-ethanolic extract of Strychnos camptoneura (longaniaceae) trunk barks in Wistar rats orld Journal of Advanced Research and Reviews, 17 (01), 386–394. |
| [27] | Dariush H. M., Bagher M., Pardis H., Paria A., Ensieh S., Parsova T., Mahmoud H., Mahmoud M., 2019. Improvement of fertility parameters with Tribulus Terrestris and Anacyclus Pyrethrum treatment in male rats. Int Braz J Urol; 45 (5): 1043-1054. |
| [28] | Maïmouna C. K., Blaise W. K, I., 2021. Effect of Different Parts of Kigelia africana Fruit Aqueous Extracts on Sperm Parameters and Testis Advances in Reproductive Sciences; 9: 171-188. |
| [29] | Chikezie., Uchechukwu N., 2017. Phytochemical and Proximate Compositions of Tetracarpidium conophorum [African Walnut] Seeds, International Journal of Research Studies in Biosciences; 5 (10): 25-31. |
| [30] | Knasmüller S., Nersesyan A., Misík M., Gerner C., Mikulits W., Ehrlich V., Hoelzl C., Szakmary A., Wagner K. H., 2008. Use of conventional and -omics based methods for health claims of dietary antioxidants: a critical overview. Br. J. Nutr; 99: 3-52. |
| [31] | Nounamo Guiekep A. J., Kenfack A., Ngoula F., Vemo, N. B., Nguemmeugne, K. S., Tedonkeng P. E.,. 2019. Attenuating effects of Mangifera indica leaves ethanolic extract against acetamiprid induced reproductive toxicity in male guinea pigs Veterinary Research Forum. 10 (3) 187-192. |
| [32] | Vemo B. N., Kenfack A., Ngoula F., Akono N. E., Kodjio N., Guiekep N. A. J., Tsambou megnimeza A. M., and Teguia A., 2017. Effects of ethanol extract of Bersama engleriana leaves on oxidative stress and reproductive parameters in male Guinea pig (Cavia porcellus) exposed to cypermethrin Int. J. Biol. Chem. Sci. 11 (5): 2243-2253. |
| [33] | Badmus J. A., Adedosu T. O., Fatoki J. O., et al., 2011. Lipid peroxidation inhibition and antiradical activities of some leaf fractions of Mangifera indica. Acta Pol Pharm; 68 (1): 23-29. |
APA Style
Herman, A., Landry, M. M., Gallo, M. D. -. B., Stéphane, W. L. B., Moukouma, C., et al. (2023). Effects of Aqueous Extract of Tetracarpidium conophorum (Mull. Arg.) & Hutch Kernels on Semen Quality and Oxidative Stress Parameters in Male Rats. American Journal of BioScience, 11(6), 164-170. https://doi.org/10.11648/j.ajbio.20231106.15
ACS Style
Herman, A.; Landry, M. M.; Gallo, M. D. -. B.; Stéphane, W. L. B.; Moukouma, C., et al. Effects of Aqueous Extract of Tetracarpidium conophorum (Mull. Arg.) & Hutch Kernels on Semen Quality and Oxidative Stress Parameters in Male Rats. Am. J. BioScience 2023, 11(6), 164-170. doi: 10.11648/j.ajbio.20231106.15
AMA Style
Herman A, Landry MM, Gallo MD-B, Stéphane WLB, Moukouma C, et al. Effects of Aqueous Extract of Tetracarpidium conophorum (Mull. Arg.) & Hutch Kernels on Semen Quality and Oxidative Stress Parameters in Male Rats. Am J BioScience. 2023;11(6):164-170. doi: 10.11648/j.ajbio.20231106.15
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Download@article{10.11648/j.ajbio.20231106.15,
author = {Akassa Herman and Miguel Martial Landry and Mongo Dieu -Merci Bevel Gallo and Wossolo Lingomo Bertrand Stéphane and Constantin Moukouma and Osandze Rachie and Arnaud Wilfrid Etou Ossibi and Ange Antoine Abena},
title = {Effects of Aqueous Extract of Tetracarpidium conophorum (Mull. Arg.) & Hutch Kernels on Semen Quality and Oxidative Stress Parameters in Male Rats},
journal = {American Journal of BioScience},
volume = {11},
number = {6},
pages = {164-170},
doi = {10.11648/j.ajbio.20231106.15},
url = {https://doi.org/10.11648/j.ajbio.20231106.15},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20231106.15},
abstract = {The aim of the present study was to evaluate the effects of the aqueous extract of Tetracarpidium conophorum kernels on semen quality and oxidative stress parameters in male rats. 20 rats were randomly divided into 4 lots of 5 animals each, and lots 1 and 2 were given distilled water and the reference molecule for 28 days, while lots 3 and 4 were treated with the aqueous extract of Tetracarpidium conophorum kernels (250 and 500 mg/kg/po). The results show that administration of aqueous extract of Tetracarpidium conophorum kernels (250 and 500 mg/kg/po) to rats produced significant dose-dependent increases (p 0.05). Administration of the aqueous extract of Tetracarpidium conophorum kernels at the doses studied resulted in no significant variation (p > 0.05) in MDA and CAT concentrations compared with distilled water. However, there was a significant increase (p Tetracarpidium conophorum aqueous extract compared to rats given distilled water. Chemical analysis of the aqueous extract of Tetracarpidium conophorum kernels revealed the presence of polyphenols and total flavonoids with anti-free radical and antioxidant effects. These results suggest that the aqueous extract of Tetracarpidium conophorum may have spermatogenic and antioxidant potential.
},
year = {2023}
}
TY - JOUR T1 - Effects of Aqueous Extract of Tetracarpidium conophorum (Mull. Arg.) & Hutch Kernels on Semen Quality and Oxidative Stress Parameters in Male Rats AU - Akassa Herman AU - Miguel Martial Landry AU - Mongo Dieu -Merci Bevel Gallo AU - Wossolo Lingomo Bertrand Stéphane AU - Constantin Moukouma AU - Osandze Rachie AU - Arnaud Wilfrid Etou Ossibi AU - Ange Antoine Abena Y1 - 2023/12/22 PY - 2023 N1 - https://doi.org/10.11648/j.ajbio.20231106.15 DO - 10.11648/j.ajbio.20231106.15 T2 - American Journal of BioScience JF - American Journal of BioScience JO - American Journal of BioScience SP - 164 EP - 170 PB - Science Publishing Group SN - 2330-0167 UR - https://doi.org/10.11648/j.ajbio.20231106.15 AB - The aim of the present study was to evaluate the effects of the aqueous extract of Tetracarpidium conophorum kernels on semen quality and oxidative stress parameters in male rats. 20 rats were randomly divided into 4 lots of 5 animals each, and lots 1 and 2 were given distilled water and the reference molecule for 28 days, while lots 3 and 4 were treated with the aqueous extract of Tetracarpidium conophorum kernels (250 and 500 mg/kg/po). The results show that administration of aqueous extract of Tetracarpidium conophorum kernels (250 and 500 mg/kg/po) to rats produced significant dose-dependent increases (p 0.05). Administration of the aqueous extract of Tetracarpidium conophorum kernels at the doses studied resulted in no significant variation (p > 0.05) in MDA and CAT concentrations compared with distilled water. However, there was a significant increase (p Tetracarpidium conophorum aqueous extract compared to rats given distilled water. Chemical analysis of the aqueous extract of Tetracarpidium conophorum kernels revealed the presence of polyphenols and total flavonoids with anti-free radical and antioxidant effects. These results suggest that the aqueous extract of Tetracarpidium conophorum may have spermatogenic and antioxidant potential. VL - 11 IS - 6 ER -
Laboratory of Biochemistry and Pharmacology, Faculty of Health Sciences, Marien Ngouabi University, Brazzaville, Republic of the Congo; Laboratory of Animal Physiology, Faculty of Science and Technology, Marien Ngouabi University, Brazzaville, Republic of the Congo
